Journal article
β-caryophyllene, a dietary cannabinoid, complexed with β-cyclodextrin produced anti-hyperalgesic effect involving the inhibition of Fos expression in superficial dorsal horn
Life sciences (1973), Vol.149, pp.34-41
03/15/2016
DOI: 10.1016/j.lfs.2016.02.049
PMID: 26883973
Abstract
Evaluate the anti-hyperalgesic effect of the complex containing β-caryophyllene (βCP) and β-cyclodextrin (βCD) in a non-inflammatory chronic muscle pain mice model and investigated its action on superficial dorsal horn of the lumbar spinal cord.
The βCP-βCD complex were prepared and characterized through the DSC, TG/DTG, FTIR, XRD and SEM. The model of chronic muscle pain was induced by two injections of pH4.0 saline (20μL) into left gastrocnemius 5days apart. After confirming hyperalgesia, male mice were treated with βCP-βCD (10 or 20mg/kg; p.o.) or vehicle (saline 0.9%, p.o.) daily for 9days. 1h after, the mechanical hyperalgesia, muscle withdrawal thresholds and motor performance were evaluated. To evaluate the βCP-βCD action on spinal cord, animals induced with chronic muscle pain were treated with βCP-βCD (20mg/kg; p.o.) or vehicle (saline 0.9%, p.o.) and 90min. after, were perfused, the lumbar spinal cord collected, crioprotected, cut and submitted in an immunofluorescence protocol for Fos protein.
The characterization tests indicated that βCP were efficiently incorporated into βCD. The oral treatment with βCP-βCD, at all doses tested, produced a significant (p<0.05) reduction on mechanical hyperalgesia and a significant (p<0.05) increase in muscle withdrawal thresholds, without produce any alteration in force. In addition, βCP-βCD was able to significantly (p<0.05) decrease Fos expression in the superficial dorsal horn.
Thus, βCP-βCD attenuates the non-inflammatory chronic muscle pain in mice and inhibits the Fos expression in the lumbar spinal cord.
Details
- Title: Subtitle
- β-caryophyllene, a dietary cannabinoid, complexed with β-cyclodextrin produced anti-hyperalgesic effect involving the inhibition of Fos expression in superficial dorsal horn
- Creators
- Lucindo J Quintans-Júnior - Department of Physiology, Federal University of Sergipe, São Cristóvão, Sergipe, BrazilAdriano A.S Araújo - Department of Pharmacy, Federal University of Sergipe, São Cristóvão, Sergipe, BrazilRenan G Brito - Department of Physiology, Federal University of Sergipe, São Cristóvão, Sergipe, BrazilPriscila L Santos - Department of Physiology, Federal University of Sergipe, São Cristóvão, Sergipe, BrazilJullyana S.S Quintans - Department of Physiology, Federal University of Sergipe, São Cristóvão, Sergipe, BrazilPaula P Menezes - Department of Pharmacy, Federal University of Sergipe, São Cristóvão, Sergipe, BrazilMairim R Serafini - Department of Pharmacy, Federal University of Sergipe, São Cristóvão, Sergipe, BrazilGabriel F Silva - Department of Food Engineering, Federal University of Sergipe, São Cristóvão, Sergipe, BrazilFlavio M.S Carvalho - Department of Mineralogy and Petrology, University of São Paulo, São Paulo, BrazilNicole K Brogden - Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, University of Iowa, Iowa City, IA, USAKathleen A Sluka - Physical Therapy and Rehabilitation Science, Carver College of Medicine, University of Iowa, Iowa City, IA, USA
- Resource Type
- Journal article
- Publication Details
- Life sciences (1973), Vol.149, pp.34-41
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.lfs.2016.02.049
- PMID
- 26883973
- ISSN
- 0024-3205
- eISSN
- 1879-0631
- Grant note
- DOI: 10.13039/501100003593, name: CNPq; DOI: 10.13039/501100005671, name: FAPITEC/SE; DOI: 10.13039/501100002322, name: Capes
- Language
- English
- Date published
- 03/15/2016
- Academic Unit
- Dermatology; Iowa Neuroscience Institute; Pharmaceutical Sciences and Experimental Therapeutics; Nursing; Physical Therapy and Rehabilitation Science; Neuroscience and Pharmacology
- Record Identifier
- 9984025469202771
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