1274 full-open reading frames of transcripts expressed in the developing mouse nervous system

Maria F Bonaldo; Thomas B Bair; Todd E Scheetz; Einat Snir; Ike Akabogu; Jennifer L Bair; Brian Berger; Keith Crouch; Aja Davis; Mari E Eyestone; Catherine Keppel; Tamara A Kucaba; Mark Lebeck; Jenny L Lin; Anna I R de Melo; Joshua Rehmann; Rebecca S Reiter; Kelly Schaefer; Christina Smith; Dylan Tack; Kurtis Trout; Val C Sheffield; Jim J-C Lin; Thomas L Casavant; Marcelo B Soares

doi:10.1101/gr.2601304

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1274 full-open reading frames of transcripts expressed in the developing mouse nervous system

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Open access

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1274 full-open reading frames of transcripts expressed in the developing mouse nervous system

Maria F Bonaldo, Thomas B Bair, Todd E Scheetz, Einat Snir, Ike Akabogu, Jennifer L Bair, Brian Berger, Keith Crouch, Aja Davis, Mari E Eyestone, …

Genome research, Vol.14(10B), pp.2053-2063

10/2004

DOI: 10.1101/gr.2601304

PMCID: PMC528920

PMID: 15489326

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Abstract

As part of the trans-National Institutes of Health (NIH) Mouse Brain Molecular Anatomy Project (BMAP), and in close coordination with the NIH Mammalian Gene Collection Program (MGC), we initiated a large-scale project to clone, identify, and sequence the complete open reading frame (ORF) of transcripts expressed in the developing mouse nervous system. Here we report the analysis of the ORF sequence of 1274 cDNAs, obtained from 47 full-length-enriched cDNA libraries, constructed by using a novel approach, herein described. cDNA libraries were derived from size-fractionated cytoplasmic mRNA isolated from brain and eye tissues obtained at several embryonic stages and postnatal days. Altogether, including the full-ORF MGC sequences derived from these libraries by the MGC sequencing team, NIH_BMAP full-ORF sequences correspond to approximately 20% of all transcripts currently represented in mouse MGC. We show that NIH_BMAP clones comprise 68% of mouse MGC cDNAs > or =5 kb, and 54% of those > or =4 kb, as of March 15, 2004. Importantly, we identified transcripts, among the 1274 full-ORF sequences, that are exclusively or predominantly expressed in brain and eye tissues, many of which encode yet uncharacterized proteins.

Pregnancy

Eye - metabolism

Brain - embryology

Brain - anatomy & histology

Gene Library

Eye - anatomy & histology

Mice, Inbred C57BL

RNA, Messenger - genetics

Gene Expression Profiling

Open Reading Frames - genetics

RNA, Messenger - metabolism

Nerve Tissue Proteins - genetics

Brain - metabolism

Eye - embryology

Animals

Nerve Tissue Proteins - classification

Female

Mice

Genome

DNA, Complementary

Gene Expression Regulation, Developmental - physiology

Details

Title: Subtitle: 1274 full-open reading frames of transcripts expressed in the developing mouse nervous system
Creators: Maria F Bonaldo - Department of Pediatrics, The University of Iowa, Iowa City, Iowa 52242, USA
Thomas B Bair
Todd E Scheetz
Einat Snir
Ike Akabogu
Jennifer L Bair
Brian Berger
Keith Crouch
Aja Davis
Mari E Eyestone
Catherine Keppel
Tamara A Kucaba
Mark Lebeck
Jenny L Lin
Anna I R de Melo
Joshua Rehmann
Rebecca S Reiter
Kelly Schaefer
Christina Smith
Dylan Tack
Kurtis Trout
Val C Sheffield
Jim J-C Lin
Thomas L Casavant
Marcelo B Soares
Resource Type: Journal article
Publication Details: Genome research, Vol.14(10B), pp.2053-2063
DOI: 10.1101/gr.2601304
PMID: 15489326
PMCID: PMC528920
NLM abbreviation: Genome Res
ISSN: 1088-9051
eISSN: 1549-5469
Publisher: United States
Grant note: F32 HG002881 / NHGRI NIH HHS 1F32HG002881-01A1 / NHGRI NIH HHS N01MH12006 / NIMH NIH HHS
Language: English
Date published: 10/2004
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Electrical and Computer Engineering; Stead Family Department of Pediatrics; Pathology; Iowa Neuroscience Institute; Medical Genetics and Genomics; Biology; Family and Community Medicine; Ophthalmology and Visual Sciences
Record Identifier: 9983980079002771

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