Journal article
17-β Estradiol Enhances Osteogenic and Adipogenic Differentiation of Human Adipose-Derived Stromal Cells
Tissue engineering, Vol.13(6), pp.1197-1203
06/2007
DOI: 10.1089/ten.2006.0317
PMID: 17518737
Abstract
Adipose-derived stromal cells (ASCs) possess multiple differentiation potentials and may serve as a cell source, if effectively modulated, for regenerative medicine and tissue engineering. Due to estrogen's function in tissue and organ development through regulating cell proliferation and differentiation, we hypothesized that an estrogen supplement may effectively enhance the multiple differentiation potentials of human ASCs. 17-β Estradiol (E2) was investigated for modulating in vitro osteogenic and adipogenic differentiation in human ASCs isolated from a healthy female donor. After ASCs' exposure to osteogenic and adipogenic differentiation medium supplemented with different concentrations of E2, osteogenic markers (alkaline phosphatase activity, extracellular matrix, calcium deposition, and osteocalcin expression) and adipogenic parameters (lipid accumulation and differentiated cell population) significantly improved. Estrogen's enhancement is dose dependent and linked to differing alpha and beta estrogen receptors. Our data preliminarily demonstrate that estrogen can modulate the differentiation, and potentially improve the efficiency of ASCs in stem cell-based tissue engineering and regeneration. However, further study is needed to verify the regulatory functions of estrogen on ASC differentiations of donors with different ages and genders. © Mary Ann Liebert, Inc.
Details
- Title: Subtitle
- 17-β Estradiol Enhances Osteogenic and Adipogenic Differentiation of Human Adipose-Derived Stromal Cells
- Creators
- Liu Hong - University of Illinois ChicagoAylin Colpan - University of Illinois ChicagoIoana A. Peptan - University of Illinois ChicagoJoseph Daw - University of Illinois ChicagoAnne George - University of Illinois ChicagoCarla A. Evans - TP Orthodontics
- Resource Type
- Journal article
- Publication Details
- Tissue engineering, Vol.13(6), pp.1197-1203
- DOI
- 10.1089/ten.2006.0317
- PMID
- 17518737
- NLM abbreviation
- Tissue Eng
- ISSN
- 1076-3279
- eISSN
- 1557-8690
- Language
- English
- Date published
- 06/2007
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Prosthodontics; Craniofacial Anomalies Research Center; Dental Research
- Record Identifier
- 9984367641102771
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