Journal article
2-(4-chlorophenyl)benzo-1,4-quinone induced ROS-signaling inhibits proliferation in human non-malignant prostate epithelial cells
Environment international, Vol.36(8), pp.924-930
11/2010
DOI: 10.1016/j.envint.2010.01.001
PMCID: PMC2888671
PMID: 20163859
Abstract
Polychlorinated biphenyls (PCBs) and their metabolites are environmental chemical contaminants which can produce reactive oxygen species (ROS) by auto-oxidation of dihydroxy PCBs as well as the reduction of quinones and redox-cycling. We investigate the hypothesis that 2-(4-chlorophenyl)benzo-1,4-quinone (4-Cl-BQ), a metabolite of 4-chlorobiphenyl (PCB3), induced ROS-signaling inhibits cellular proliferation. Monolayer cultures of exponentially growing asynchronous human non-malignant prostate epithelial cells (RWPE-1) were incubated with 0–6 micromolar of 4-Cl-BQ and harvested at the end of 72 h of incubation to assess antioxidant enzyme expression, cellular ROS levels, cell growth, and cell cycle phase distributions. 4-Cl-BQ decreased manganese superoxide dismutase (MnSOD) activity, protein, and mRNA levels. 4-Cl-BQ treatment increased dihydroethidium (DHE) fluorescence, which was suppressed in cells pre-treated with polyethylene glycol conjugated superoxide dismutase (PEG-SOD). The increase in ROS levels was associated with a decrease in cell growth, and an increase in the percentage of S-phase cells. These effects were suppressed in cells pretreated with PEG-SOD. 4-Cl-BQ treatment did not change the protein levels of phosphorylated H2AX at the end of 72 h of incubation, suggesting that the inhibition in cell growth and accumulation of cells in S-phase at the end of the treatments were probably not due to 4-Cl-BQ induced DNA double strand break. These results demonstrate that MnSOD activity and ROS-signaling perturb proliferation in 4-Cl-BQ treated
in vitro
cultures of human prostate cells.
Details
- Title: Subtitle
- 2-(4-chlorophenyl)benzo-1,4-quinone induced ROS-signaling inhibits proliferation in human non-malignant prostate epithelial cells
- Creators
- Leena Chaudhuri - Free Radical and Radiation Biology Program, Department of Radiation Oncology, University of Iowa, Iowa City, IowaEhab H Sarsour - Free Radical and Radiation Biology Program, Department of Radiation Oncology, University of Iowa, Iowa City, IowaPrabhat C Goswami - Free Radical and Radiation Biology Program, Department of Radiation Oncology, University of Iowa, Iowa City, Iowa
- Resource Type
- Journal article
- Publication Details
- Environment international, Vol.36(8), pp.924-930
- DOI
- 10.1016/j.envint.2010.01.001
- PMID
- 20163859
- PMCID
- PMC2888671
- NLM abbreviation
- Environ Int
- ISSN
- 0160-4120
- eISSN
- 1873-6750
- Language
- English
- Date published
- 11/2010
- Academic Unit
- Radiation Oncology
- Record Identifier
- 9984047747002771
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