Journal article
25-Hydroxyvitamin D deficiency is associated with inflammation-linked vascular endothelial dysfunction in middle-aged and older adults
Hypertension (Dallas, Tex. 1979), Vol.57(1), pp.63-69
11/29/2010
DOI: 10.1161/HYPERTENSIONAHA.110.160929
PMCID: PMC3020150
PMID: 21115878
Abstract
We tested the hypothesis that vascular endothelial function, assessed by endothelium-dependent dilation, is related to serum vitamin D status among middle-aged and older adults without clinical disease, and that this is linked to inflammation. Brachial artery flow-mediated dilation (FMD), a measure of endothelium-dependent dilation, was lower (P<0.01) in vitamin D insufficient (3.7±0.2%; serum 25-hydroxyvitamin D [25(OH)D]: 20-29ng/mL, 62±1yr, n=31, mean±S.E.) and deficient (3.2±0.3%; 25(OH)D: <20ng/mL, 63±2 yr, n=22) vs. sufficient (4.6±0.4%; 25(OH)D: >29ng/mL; 61±1yr, n=22) subjects, whereas endothelium-independent dilation (brachial dilation to sublingual nitroglycerine) did not differ (P=0.45). Among all subjects, brachial FMD was positively related to serum 25(OH)D (%Δ: r=0.35, P<0.01), but not 1,25-dihydroxyvitamin D (r=-0.06, P=0.61), the active form of vitamin D. Vascular endothelial cell expression of the pro-inflammatory transcription factor nuclear factor κB (NFκB) was greater in deficient vs. sufficient subjects (0.59±0.07 vs. 0.44±0.05, P<0.05), and inhibition of NFκB (4d oral salsalate) improved FMD to a greater extent in subjects with lower vs. higher 25(OH)D (+3.7±0.6 vs. +2.0±0.2%, P<0.05). Endothelial cell expression of the downstream pro-inflammatory cytokine interleukin 6 also was higher in deficient vs. sufficient subjects (0.67±0.08 vs. 0.47±0.05, P<0.01) and inversely related to serum 25(OH)D level (r=-0.62; P<0.01), whereas vitamin D receptor and 1-α hydroxylase, the 25(OH)D to 1,25-dihydroxyvitamin D converting enzyme, were lower (P<0.05). Inadequate serum 25(OH)D is associated with vascular endothelial dysfunction among healthy middle-aged/older adults and this is mediated in part by NFκB-related inflammation. Reduced vitamin D receptor and 1-α hydroxylase may be molecular mechanisms linking vitamin D insufficiency to endothelial dysfunction.
Details
- Title: Subtitle
- 25-Hydroxyvitamin D deficiency is associated with inflammation-linked vascular endothelial dysfunction in middle-aged and older adults
- Creators
- Kristen L Jablonski - University of Colorado BoulderMichel Chonchol - University of Colorado BoulderGary L Pierce - University of Colorado BoulderAshley E Walker - University of Colorado BoulderDouglas R Seals - University of Colorado Boulder
- Resource Type
- Journal article
- Publication Details
- Hypertension (Dallas, Tex. 1979), Vol.57(1), pp.63-69
- DOI
- 10.1161/HYPERTENSIONAHA.110.160929
- PMID
- 21115878
- PMCID
- PMC3020150
- ISSN
- 0194-911X
- eISSN
- 1524-4563
- Language
- English
- Date published
- 11/29/2010
- Academic Unit
- Health and Human Physiology; Internal Medicine
- Record Identifier
- 9984267249002771
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