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2D IR spectroscopy using four-wave mixing, pulse shaping, and IR upconversion: a quantitative comparison
Journal article   Peer reviewed

2D IR spectroscopy using four-wave mixing, pulse shaping, and IR upconversion: a quantitative comparison

William Rock, Yun-Liang Li, Philip Pagano and Christopher M Cheatum
The journal of physical chemistry. A, Molecules, spectroscopy, kinetics, environment, & general theory, Vol.117(29), pp.6073-6083
07/25/2013
DOI: 10.1021/jp312817t
PMCID: PMC3723749
PMID: 23687988
url
http://doi.org/10.1021/jp312817tView
Open Access

Abstract

Recent technological advances have led to major changes in the apparatuses used to collect 2D IR spectra. Pulse shaping offers several advantages including rapid data collection, inherent phase stability, and phase-cycling capabilities. Visible array detection via upconversion allows the use of visible detectors that are cheaper, faster, more sensitive, and less noisy than IR detectors. However, despite these advantages, many researchers are reluctant to implement these technologies. Here we present a quantitative study of the S/N of 2D IR spectra collected with a traditional four-wave mixing (FWM) apparatus, with a pulse shaping apparatus, and with visible detection via upconversion to address the question of whether weak chromophores at low concentrations are still accessible with such an apparatus. We find that the enhanced averaging capability of the pulse shaping apparatus enables the detection of small signals that would be challenging to measure even with the traditional FWM apparatus, and we demonstrate this ability on a sample of cyanylated dihydrofolate reductase.
 Absorption Spectrophotometry, Infrared - methods Tetrahydrofolate Dehydrogenase - chemistry Signal-To-Noise Ratio Cyanates - chemistry Cysteine - chemistry Dimethylformamide - chemistry

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