3D Bioprinted Ferret Mesenchymal Stem Cell-Laden Cartilage Grafts for Laryngotracheal Reconstruction in a Ferret Surgical Model

Alexandra McMillan; Matthew R. Hoffman; Yan Xu; Zongliang Wu; Emma Thayer; Adreann Peel; C. Allan Guymon; Sohit Kanotra; Aliasger Salem

doi:10.1039/D4BM01251H

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3D Bioprinted Ferret Mesenchymal Stem Cell-Laden Cartilage Grafts for Laryngotracheal Reconstruction in a Ferret Surgical Model

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3D Bioprinted Ferret Mesenchymal Stem Cell-Laden Cartilage Grafts for Laryngotracheal Reconstruction in a Ferret Surgical Model

Alexandra McMillan, Matthew R. Hoffman, Yan Xu, Zongliang Wu, Emma Thayer, Adreann Peel, C. Allan Guymon, Sohit Kanotra and Aliasger Salem

Biomaterials science, Vol.13(5), pp.1304-1322

01/22/2025

DOI: 10.1039/D4BM01251H

PMCID: PMC11784027

PMID: 39886992

Appears in UI Libraries Support Open Access

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Abstract

Chondrogenic differentiation of mesenchymal stem cells (MSCs) within a three-dimensional (3D) environment can be guided to form cartilage-like tissue in vitro to generate cartilage grafts for implantation. 3D bioprinted, MSC-populated cartilage grafts have the potential to replace autologous cartilage in reconstructive airway surgery. Here, bone marrow-derived ferret MSCs (fMSCs) capable of directed musculoskeletal differentiation were generated for the first time. A multi-material, 3D bioprinted fMSC-laden scaffold was then engineered that was capable of in vitro cartilage regeneration, as evidenced by glycosaminoglycan (GAG) production and collagen II immunohistochemical staining. In vivo implantation of these 3D bioprinted scaffolds in a ferret model of laryngotracheal reconstruction (LTR) demonstrated healing of the defect site, epithelial mucosalization of the inner lumen, and expansion of the airway volume. While the implanted scaffold allowed for reconstruction of the created airway defect, minimal chondrocytes were identified at the implant site. Nevertheless, we have established the ferret as biomedical research model for airway reconstruction and, although further evaluation is warranted, the generation of fMSCs provides an opportunity for realizing the potential for 3D bioprinted regenerative stem cell platforms in the ferret.

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Title: Subtitle: 3D Bioprinted Ferret Mesenchymal Stem Cell-Laden Cartilage Grafts for Laryngotracheal Reconstruction in a Ferret Surgical Model
Creators: Alexandra McMillan - University of Iowa
Matthew R. Hoffman - University of Iowa
Yan Xu - University of Iowa
Zongliang Wu - University of Iowa
Emma Thayer - University of Iowa
Adreann Peel - University of Iowa
C. Allan Guymon - University of Iowa
Sohit Kanotra - University of Iowa
Aliasger Salem - University of Iowa
Resource Type: Journal article
Publication Details: Biomaterials science, Vol.13(5), pp.1304-1322
DOI: 10.1039/D4BM01251H
PMID: 39886992
PMCID: PMC11784027
NLM abbreviation: Biomater Sci
ISSN: 2047-4830
eISSN: 2047-4849
Publisher: Royal Society of Chemistry
Language: English
Date published: 01/22/2025
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Communication Sciences and Disorders; Stead Family Department of Pediatrics; Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering; Otolaryngology
Record Identifier: 9984775021602771

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