Journal article
3D Bioprinting of Oxygenated Cell‐Laden Gelatin Methacryloyl Constructs
Advanced healthcare materials, Vol.9(15), 1901794
08/05/2020
DOI: 10.1002/adhm.201901794
PMCID: PMC7500045
PMID: 32548961
Abstract
Cell survival during the early stages of transplantation and before new blood vessels formation is a major challenge in translational applications of 3D bioprinted tissues. Supplementing oxygen (O2) to transplanted cells via an O2 generating source such as calcium peroxide (CPO) is an attractive approach to ensure cell viability. Calcium peroxide also produces calcium hydroxide that reduces the viscosity of bioinks, which is a limiting factor for bioprinting. Therefore, adapting this solution into 3D bioprinting is of significant importance. In this study, a gelatin methacryloyl (GelMA) bioink that is optimized in terms of pH and viscosity is developed. The improved rheological properties lead to the production of a robust bioink suitable for 3D bioprinting and controlled O2 release. In addition, O2 release, bioprinting conditions, and mechanical performance of hydrogels having different CPO concentrations are characterized. As a proof of concept study, fibroblasts and cardiomyocytes are bioprinted using CPO containing GelMA bioink. Viability and metabolic activity of printed cells are checked after 7 days of culture under hypoxic condition. The results show that the addition of CPO improves the metabolic activity and viability of cells in bioprinted constructs under hypoxic condition.
This study presents a novel approach to develop oxygenated bioinks, using oxygen generating calcium peroxide particles. Cardiomyocyte‐laden oxygenated and 3D printed tissue constructs developed in this study can significantly support cell survival when applied in hypoxic conditions. They can also be delivered using minimally invasive approach to ischemic hearts.
Details
- Title: Subtitle
- 3D Bioprinting of Oxygenated Cell‐Laden Gelatin Methacryloyl Constructs
- Creators
- Ahmet Erdem - University of California, Los AngelesMohammad Ali Darabi - University of California, Los AngelesRohollah Nasiri - University of California, Los AngelesSivakoti Sangabathuni - University of California, Los AngelesYavuz Nuri Ertas - Erciyes UniversityHalima Alem - University of California, Los AngelesVahid Hosseini - University of California, Los AngelesAmir Shamloo - Sharif University of TechnologyAli S. Nasr - University of IowaSamad Ahadian - University of California, Los AngelesMehmet R. Dokmeci - Center for Minimally Invasive Therapeutics (C-MIT) University of California Los Angeles CA 90095 USAAli Khademhosseini - Center for Minimally Invasive Therapeutics (C-MIT) University of California Los Angeles CA 90095 USANureddin Ashammakhi - University of California System
- Resource Type
- Journal article
- Publication Details
- Advanced healthcare materials, Vol.9(15), 1901794
- DOI
- 10.1002/adhm.201901794
- PMID
- 32548961
- PMCID
- PMC7500045
- NLM abbreviation
- Adv Healthc Mater
- ISSN
- 2192-2640
- eISSN
- 2192-2659
- Number of pages
- 12
- Grant note
- National Institutes of Health (1R01EB021857; R01GM126571) National Foundation Postdoctoral Fellowship (P2EZP2_18798) American Heart Association (18TPA34230036; TUBITAK‐2219; 1059B191700093)
- Language
- English
- Date published
- 08/05/2020
- Academic Unit
- Surgery
- Record Identifier
- 9984322943602771
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