3D Patterning of cells in Magnetic Scaffolds for Tissue Engineering

V Goranov; T Shelyakova; R De Santis; Y Haranava; A Makhaniok; A Gloria; A Tampieri; A Russo; E Kon; M Marcacci; L Ambrosio; V A Dediu

doi:10.1038/s41598-020-58738-5

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3D Patterning of cells in Magnetic Scaffolds for Tissue Engineering

Journal article

Open access

Peer reviewed

3D Patterning of cells in Magnetic Scaffolds for Tissue Engineering

V Goranov, T Shelyakova, R De Santis, Y Haranava, A Makhaniok, A Gloria, A Tampieri, A Russo, E Kon, M Marcacci, …

Scientific reports, Vol.10(1), pp.2289-2289

02/10/2020

DOI: 10.1038/s41598-020-58738-5

PMCID: PMC7010825

PMID: 32041994

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Abstract

A three dimensional magnetic patterning of two cell types was realised in vitro inside an additive manufactured magnetic scaffold, as a conceptual precursor for the vascularised tissue. The realisation of separate arrangements of vascular and osteoprogenitor cells, labelled with biocompatible magnetic nanoparticles, was established on the opposite sides of the scaffold fibres under the effect of non-homogeneous magnetic gradients and loading magnetic configuration. The magnetisation of the scaffold amplified the guiding effects by an additional trapping of cells due to short range magnetic forces. The mathematical modelling confirmed the strong enhancement of the magnetic gradients and their particular geometrical distribution near the fibres, defining the preferential cell positioning on the micro-scale. The manipulation of cells inside suitably designed magnetic scaffolds represents a unique solution for the assembling of cellular constructs organised in biologically adequate arrangements.

Osteogenesis - physiology

Tissue Engineering - methods

Models, Chemical

Biocompatible Materials - chemistry

Humans

Mesenchymal Stem Cells - physiology

Materials Testing

Bone Regeneration

Proof of Concept Study

Tissue Scaffolds - chemistry

Neovascularization, Physiologic - physiology

Human Umbilical Vein Endothelial Cells - physiology

Magnetite Nanoparticles - chemistry

Models, Biological

Computer Simulation

Nanomedicine - methods

Magnetic Fields

Details

Title: Subtitle: 3D Patterning of cells in Magnetic Scaffolds for Tissue Engineering
Creators: V Goranov - BioDevice Systems, Praha 10, Vršovice, Bulharská, 996/20, Czech Republic. biodevicesystems@gmail.com
T Shelyakova - IRCCS Istituto Ortopedico Rizzoli, Via di Barbiano 1/10, 40136, Bologna, Italy. tatiana.shelyakova@ior.it
R De Santis - Institute of Polymers, Composites and Biomaterials, CNR-IPCB, V.le J.F. Kennedy 54 - Pad. 20 Mostra d'Oltremare, 80125, Naples, Italy
Y Haranava - BioDevice Systems, Praha 10, Vršovice, Bulharská, 996/20, Czech Republic
A Makhaniok - BioDevice Systems, Praha 10, Vršovice, Bulharská, 996/20, Czech Republic
A Gloria - Institute of Polymers, Composites and Biomaterials, CNR-IPCB, V.le J.F. Kennedy 54 - Pad. 20 Mostra d'Oltremare, 80125, Naples, Italy
A Tampieri - Institute of Science and Technology for Ceramics, CNR-ISTEC, Via Granarolo 64, 48018, Faenza, Italy
A Russo - IRCCS Istituto Ortopedico Rizzoli, Via di Barbiano 1/10, 40136, Bologna, Italy
E Kon - First Moscow State Medical University (Sechenov University), Moscow, Russian Federation
M Marcacci - Humanitas Clinical and Research Center, Via Manzoni 56, 20089, Rozzano - Milan, Italy
L Ambrosio - Institute of Polymers, Composites and Biomaterials, CNR-IPCB, V.le J.F. Kennedy 54 - Pad. 20 Mostra d'Oltremare, 80125, Naples, Italy
V A Dediu - Institute for Nanostructured Materials, CNR-ISMN, Via Gobetti 101, 40129, Bologna, Italy. valentin.dediu@cnr.it
Resource Type: Journal article
Publication Details: Scientific reports, Vol.10(1), pp.2289-2289
DOI: 10.1038/s41598-020-58738-5
PMID: 32041994
PMCID: PMC7010825
NLM abbreviation: Sci Rep
ISSN: 2045-2322
eISSN: 2045-2322
Publisher: England
Language: English
Date published: 02/10/2020
Academic Unit: Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute; Craniofacial Anomalies Research Center
Record Identifier: 9984072073402771

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