Journal article
3D Printing of Silk Protein Structures by Aqueous Solvent-Directed Molecular Assembly
Macromolecular bioscience, Vol.20(1), pp.e1900191-8
01/2020
DOI: 10.1002/mabi.201900191
PMCID: PMC6980242
PMID: 31433126
Abstract
Hierarchical molecular assembly is a fundamental strategy for manufacturing protein structures in nature. However, to translate this natural strategy into advanced digital manufacturing like three-dimensional (3D) printing remains a technical challenge. This work presents a 3D printing technique with silk fibroin to address this challenge, by rationally designing an aqueous salt bath capable of directing the hierarchical assembly of the protein molecules. This technique, conducted under aqueous and ambient conditions, results in 3D proteinaceous architectures characterized by intrinsic biocompatibility/biodegradability and robust mechanical features. The versatility of this method is shown in a diversity of 3D shapes and a range of functional components integrated into the 3D prints. The manufacturing capability is exemplified by the single-step construction of perfusable microfluidic chips which eliminates the use of supporting or sacrificial materials. The 3D shaping capability of the protein material can benefit a multitude of biomedical devices, from drug delivery to surgical implants to tissue scaffolds. This work also provides insights into the recapitulation of solvent-directed hierarchical molecular assembly for artificial manufacturing.
Details
- Title: Subtitle
- 3D Printing of Silk Protein Structures by Aqueous Solvent-Directed Molecular Assembly
- Creators
- Xuan Mu - Tufts UniversityYu Wang - Tufts UniversityChengchen Guo - Tufts UniversityYamin Li - Tufts UniversityShengjie Ling - Tufts UniversityWenwen Huang - Tufts UniversityPeggy Cebe - Tufts UniversityHuan-Hsuan Hsu - Tufts UniversityFabio De Ferrari - Tufts UniversityXiaocheng Jiang - Tufts UniversityQiaobing Xu - Tufts UniversityAlessandra Balduini - Tufts UniversityFiorenzo G Omenetto - Tufts UniversityDavid L Kaplan - Tufts University
- Resource Type
- Journal article
- Publication Details
- Macromolecular bioscience, Vol.20(1), pp.e1900191-8
- DOI
- 10.1002/mabi.201900191
- PMID
- 31433126
- PMCID
- PMC6980242
- NLM abbreviation
- Macromol Biosci
- ISSN
- 1616-5187
- eISSN
- 1616-5195
- Grant note
- R01 EB016041 / NIBIB NIH HHS U01 EB014976 / NIBIB NIH HHS P41 EB027062 / NIBIB NIH HHS R01 AR068048 / NIAMS NIH HHS R01 NS092847 / NINDS NIH HHS P41 EB002520 / NIBIB NIH HHS
- Language
- English
- Date published
- 01/2020
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering
- Record Identifier
- 9984277265602771
Metrics
26 Record Views