Journal article
3D reconstruction and comparison of shapes of DNA minicircles observed by cryo-electron microscopy
Nucleic acids research, Vol.34(18), pp.e125-e125
10/2006
DOI: 10.1093/nar/gkl675
PMCID: PMC1635295
PMID: 17012274
Abstract
We use cryo-electron microscopy to compare 3D shapes of 158 bp long DNA minicircles that differ only in the sequence within an 18 bp block containing either a TATA box or a catabolite activator protein binding site. We present a sorting algorithm that correlates the reconstructed shapes and groups them into distinct categories. We conclude that the presence of the TATA box sequence, which is believed to be easily bent, does not significantly affect the observed shapes.
Details
- Title: Subtitle
- 3D reconstruction and comparison of shapes of DNA minicircles observed by cryo-electron microscopy
- Creators
- Arnaud AmzallagCédric VaillantMathews JacobMichael UnserJan BednarJason D KahnJacques DubochetAndrzej StasiakJohn H Maddocks
- Resource Type
- Journal article
- Publication Details
- Nucleic acids research, Vol.34(18), pp.e125-e125
- DOI
- 10.1093/nar/gkl675
- PMID
- 17012274
- PMCID
- PMC1635295
- NLM abbreviation
- Nucleic Acids Res
- ISSN
- 0305-1048
- eISSN
- 1362-4962
- Publisher
- Oxford University Press
- Language
- English
- Date published
- 10/2006
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Radiology; Electrical and Computer Engineering; Iowa Neuroscience Institute; Radiation Oncology
- Record Identifier
- 9984070984802771
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