Journal article
5-Fluoro-orotic acid induces chromosome alterations in Candida albicans
Yeast (Chichester, England), Vol.22(1), pp.57-70
01/15/2005
DOI: 10.1002/yea.1191
PMID: 15635674
Abstract
Treatment of a prototrophic laboratory strain of Candida albicans with 5-fluoro-orotic acid (5-FOA) produced two major types of mutants with chromosomal alterations, 5-FOA-resistant (FoaR) and those remaining sensitive (FoaS). Both major types remained Ura+. FoaR mutants, produced after a long exposure, contained either a duplication of chromosome 4b or an inner enlargement of chromosome 5b. The average mutant frequency was approximately 1.0 x 10(-5). The reverse mutation of FoaR to FoaS also caused the loss of either the extra chromosome 4b or the enlarged chromosome 5b, revealing a causal relationship between the resistance and the specific chromosome constitution. The cells remained sensitive after a relatively short 24 h exposure to 5-FOA medium, but the treatment induced non-specific changes in lengths of various chromosomes. Furthermore, FoaR type mutants acquired a notable chromosomal and phenotypic instability. Our results indicate the necessity of electrokaryotyping of strains that have been exposed to 5-FOA, especially with studies of gene function and with DNA microarray assays.
Details
- Title: Subtitle
- 5-Fluoro-orotic acid induces chromosome alterations in Candida albicans
- Creators
- Melanie Wellington - Department of Pediatrics, University of Rochester Medical Center, Rochester, NY 14642, USAElena Rustchenko
- Resource Type
- Journal article
- Publication Details
- Yeast (Chichester, England), Vol.22(1), pp.57-70
- DOI
- 10.1002/yea.1191
- PMID
- 15635674
- ISSN
- 0749-503X
- eISSN
- 1097-0061
- Grant note
- T32 AI 07464 / NIAID NIH HHS AI29433 / NIAID NIH HHS
- Language
- English
- Date published
- 01/15/2005
- Academic Unit
- Stead Family Department of Pediatrics; Infectious Disease (Pediatrics)
- Record Identifier
- 9984093351102771
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