Journal article
5-fluoro-orotic acid induces chromosome alterations in genetically manipulated strains of Candida albicans
Mycologia, Vol.98(3), pp.393-398
05/01/2006
DOI: 10.1080/15572536.2006.11832674
PMID: 17040068
Abstract
We previously reported the occurrence of chromosome alterations in a Candida albicans prototrophic strain 3153A treated with 5-fluoro-orotic acid (5-FOA). In this study we investigated the mutagenic properties of 5-FOA with two derivatives of C. albicans strain CAF4-2 (ura3/ura3), each containing an ectopic copy of URA3 gene (ura3/ ura3 URA3) on a different chromosome. As expected, after the ura3/ura3 URA3 constructs were applied to 5-FOA containing solid medium, the "pop-outs" that lost URA3 appeared. However most of the "pop-outs" acquired various chromosome alterations. Thus constructs exposed to 5-FOA should be examined for chromosome alterations or the use of 5-FOA should be avoided.
Details
- Title: Subtitle
- 5-fluoro-orotic acid induces chromosome alterations in genetically manipulated strains of Candida albicans
- Creators
- Melanie WellingtonM AnaulKabir Elena Rustchenko
- Resource Type
- Journal article
- Publication Details
- Mycologia, Vol.98(3), pp.393-398
- Publisher
- Taylor & Francis
- DOI
- 10.1080/15572536.2006.11832674
- PMID
- 17040068
- ISSN
- 0027-5514
- eISSN
- 1557-2536
- Language
- English
- Date published
- 05/01/2006
- Academic Unit
- Stead Family Department of Pediatrics; Infectious Disease (Pediatrics)
- Record Identifier
- 9984093481002771
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