5' proximal regions of Arabidopsis nitrate reductase genes direct nitrate-induced transcription in transgenic tobacco

Y Lin; C F Hwang; J B Brown; C L Cheng

doi:10.1104/pp.106.2.477

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5' proximal regions of Arabidopsis nitrate reductase genes direct nitrate-induced transcription in transgenic tobacco

Journal article

Open access

5' proximal regions of Arabidopsis nitrate reductase genes direct nitrate-induced transcription in transgenic tobacco

Y Lin, C F Hwang, J B Brown and C L Cheng

Plant physiology (Bethesda), Vol.106(2), pp.477-484

10/1994

DOI: 10.1104/pp.106.2.477

PMCID: PMC159552

PMID: 7991680

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Abstract

Nitrate reductase (NR) is the first enzyme in nitrate assimilation, a critical process for plant survival. The regulation of NR gene expression is complex, involving both internal and external factors. Of these, nitrate induction of NR gene expression has been studied most extensively and is well conserved among bacteria, fungi, and higher plants. We are interested in understanding the mechanism of nitrate induction of higher plant NR genes. Here we describe promoter analyses of the 5' flanking regions of the Arabidopsis NR genes, NR1 and NR2, with respect to nitrate induction of gene expression. To facilitate these analyses, a nitrate induction procedure using T1 transgenic tobacco plants was established. Approximately 1.5-kb 5' flanking regions of the two Arabidopsis NR genes (NR1 and NR2) were fused to a reporter gene and its expression in transgenic plants was analyzed. Deletion analyses of these regions show that 238- and 188-bp 5' flanking regions of the NR1 and NR2, respectively, contain sequences responsive to nitrate induction.

Arabidopsis - enzymology

Arabidopsis - genetics

Base Sequence

Chloramphenicol O-Acetyltransferase - biosynthesis

Gene Expression

Genes, Regulator

Molecular Sequence Data

Nitrate Reductase

Nitrate Reductases - genetics

Nitrates - pharmacology

Plants, Genetically Modified

Plants, Toxic

Recombinant Fusion Proteins - biosynthesis

Regulatory Sequences, Nucleic Acid

Sequence Deletion

Tobacco - metabolism

Transcription, Genetic - drug effects

Details

Title: Subtitle: 5' proximal regions of Arabidopsis nitrate reductase genes direct nitrate-induced transcription in transgenic tobacco
Creators: Y Lin - Department of Biological Sciences, University of Iowa, Iowa City 52242
C F Hwang
J B Brown
C L Cheng
Resource Type: Journal article
Publication Details: Plant physiology (Bethesda), Vol.106(2), pp.477-484
DOI: 10.1104/pp.106.2.477
PMID: 7991680
PMCID: PMC159552
NLM abbreviation: Plant Physiol
ISSN: 0032-0889
eISSN: 1532-2548
Grant note: RP07035-25 / PHS HHS GM07091 / NIGMS NIH HHS GM46116 / NIGMS NIH HHS
Language: English
Date published: 10/1994
Academic Unit: Biology
Record Identifier: 9984217426502771

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