Journal article
7SK snRNA: a noncoding RNA that plays a major role in regulating eukaryotic transcription
Wiley interdisciplinary reviews. RNA, Vol.3(1), pp.92-103
01/2012
DOI: 10.1002/wrna.106
PMCID: PMC3223291
PMID: 21853533
Abstract
The human 7SK small nuclear RNA (snRNA) is an abundant noncoding RNA whose function has been conserved in evolution from invertebrates to humans. It is transcribed by RNA polymerase III (RNAPIII) and is located in the nucleus. Together with associated cellular proteins, 7SK snRNA regulates the activity of the positive transcription elongation factor, P-TEFb. In humans, this regulation is accomplished by the recruitment of P-TEFb by the 7SK snRNA-binding proteins, HEXIM1 or HEXIM2, which inhibit the kinase activity of P-TEFb. P-TEFb regulates the transition of promoter proximally paused RNA polymerase II (RNAPII) into productive elongation, thereby, allowing efficient mRNA production. The protein composition of the 7SK small nuclear ribonucleoprotein (snRNP) is regulated dynamically. Whereas the La related protein LARP7 is a constitutive component, the methyl phosphate capping enzyme MePCE associates secondarily to phosphorylate the 5' end of 7SK snRNA. The release of active P-TEFb is closely followed by release of HEXIM proteins and both are replaced by heterogeneous nuclear ribonucleoproteins (hnRNPs). The released P-TEFb activates the expression of most cellular and viral genes. Regulated release of P-TEFb determines the expression pattern of many of the genes that respond to environmental stimuli and regulate growth, proliferation and differentiation of cells.
Details
- Title: Subtitle
- 7SK snRNA: a noncoding RNA that plays a major role in regulating eukaryotic transcription
- Creators
- B. Matija Peterlin - Departments of Medicine, Microbiology and Immunology, Rosalind Russel Medical Research Center, University of California, San Francisco, CA 94143-0703, USA. Department of Virology, Haartman Institute, University of Helsinki, Haartmaninkatu 3, 00290 Helsinki, FinlandJohn E Brogie - Biochemistry Department, University of Iowa, Iowa City, IO 52242, USADavid H Price - Biochemistry Department, University of Iowa, Iowa City, IO 52242, USA
- Resource Type
- Journal article
- Publication Details
- Wiley interdisciplinary reviews. RNA, Vol.3(1), pp.92-103
- DOI
- 10.1002/wrna.106
- PMID
- 21853533
- PMCID
- PMC3223291
- NLM abbreviation
- Wiley Interdiscip Rev RNA
- ISSN
- 1757-7004
- eISSN
- 1757-7012
- Grant note
- R01 AI054340-04 || AI / National Institute of Allergy and Infectious Diseases Extramural Activities : NIAID R33 AI074392-05 || AI / National Institute of Allergy and Infectious Diseases Extramural Activities : NIAID R01 GM035500-27 || GM / National Institute of General Medical Sciences : NIGMS
- Language
- English
- Date published
- 01/2012
- Academic Unit
- Biochemistry and Molecular Biology
- Record Identifier
- 9984024515802771
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