A 45-kDa protein antigenically related to band 3 is selectively expressed in kidney mitochondria

Lynda S Ostedgaard; Michael L Jennings; Lawrence P Karniski; Victor L Schuster

doi:10.1073/pnas.88.3.981

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A 45-kDa protein antigenically related to band 3 is selectively expressed in kidney mitochondria

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A 45-kDa protein antigenically related to band 3 is selectively expressed in kidney mitochondria

Lynda S Ostedgaard, Michael L Jennings, Lawrence P Karniski and Victor L Schuster

Proceedings of the National Academy of Sciences - PNAS, Vol.88(3), pp.981-985

02/01/1991

DOI: 10.1073/pnas.88.3.981

PMCID: PMC50938

PMID: 1704136

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Abstract

Anion exchange similar to that catalyzed by erythrocyte band 3 occurs across many nonerythroid cell membranes. To identify anion-exchange proteins structurally related to band 3, we immunoblotted rabbit kidney medullary membrane fractions with anti-band 3 antibodies. Immunoblots using antibodies to the cytoplasmic domain of band 3 revealed cross-reactive proteins in the plasma membrane fraction only. In contrast, two monoclonal antibodies against band 3 membrane domain labeled a 45-kDa protein; further immunoblotting and immunogold studies of membrane fractions and kidney sections using one of the anti-membrane domain antibodies showed that labeling was strongest in mitochondria of H(+)-secreting collecting duct cells. Tissue-to-tissue expression of the 45-kDa mitochondrial protein was variable: kidney medulla greater than heart greater than kidney cortex much greater than liver. We conclude that a 45-kDa protein with immunological cross-reactivity to the erythrocyte band 3 membrane domain is expressed in mitochondria in a highly cell-specific fashion and speculate that the protein may play a role in mitochondrial anion transport.

Details

Title: Subtitle: A 45-kDa protein antigenically related to band 3 is selectively expressed in kidney mitochondria
Creators: Lynda S Ostedgaard - Department of Medicine, University of Iowa, Iowa City 52242
Michael L Jennings - Department of Medicine, University of Iowa, Iowa City 52242
Lawrence P Karniski - Department of Medicine, University of Iowa, Iowa City 52242
Victor L Schuster - Department of Medicine, University of Iowa, Iowa City 52242
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.88(3), pp.981-985
DOI: 10.1073/pnas.88.3.981
PMID: 1704136
PMCID: PMC50938
NLM abbreviation: Proc Natl Acad Sci U S A
ISSN: 0027-8424
eISSN: 1091-6490
Publisher: National Academy of Sciences
Language: English
Date published: 02/01/1991
Academic Unit: Nephrology; Internal Medicine
Record Identifier: 9984094765702771

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