Journal article
A Chimeric Type 2 Adenovirus Vector with a Type 17 Fiber Enhances Gene Transfer to Human Airway Epithelia
Journal of virology, Vol.73(10), pp.8689-8695
10/1999
DOI: 10.1128/JVI.73.10.8689-8695.1999
PMCID: PMC112889
PMID: 10482622
Abstract
In studies of the genetic disease cystic fibrosis, recombinant adenovirus type 2 (Ad2) and Ad5 are being investigated as vectors to transfer cystic fibrosis transmembrane conductance regulator cDNA to airway epithelia. However, earlier work has shown that human airway epithelia are resistant to infection by Ad2 and Ad5. Therefore, we examined the efficiency of other adenovirus serotypes at infecting airway epithelia. We found that several serotypes of adenoviruses, in particular, wild-type Ad17, infected a greater number of cells than wild-type Ad2. The increased efficiency of wild-type Ad17 could be explained by increased fiber-dependent binding to the epithelia. Therefore, we constructed a chimeric virus, Ad2(17f)/βGal-2, which is identical to Ad2/βGal-2 with the exception of having the fiber protein of Ad17 replace Ad2 fiber. This vector retained the increased binding and efficiency of gene transfer to well-differentiated human airway epithelia. These data suggest that inclusion of Ad17 fiber into adenovirus vectors may improve the outlook for gene delivery to human airway epithelia.
Details
- Title: Subtitle
- A Chimeric Type 2 Adenovirus Vector with a Type 17 Fiber Enhances Gene Transfer to Human Airway Epithelia
- Creators
- Joseph Zabner - Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, Iowa 52242Miguel Chillon - Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, Iowa 52242Teresa Grunst - Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, Iowa 52242Thomas O Moninger - Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, Iowa 52242Beverly L Davidson - Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, Iowa 52242Richard Gregory - Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, Iowa 52242Donna Armentano - Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, Iowa 52242
- Resource Type
- Journal article
- Publication Details
- Journal of virology, Vol.73(10), pp.8689-8695
- Publisher
- American Society for Microbiology
- DOI
- 10.1128/JVI.73.10.8689-8695.1999
- PMID
- 10482622
- PMCID
- PMC112889
- ISSN
- 0022-538X
- eISSN
- 1098-5514
- Language
- English
- Date published
- 10/1999
- Academic Unit
- Pulmonary, Critical Care, and Occupational Medicine; Internal Medicine
- Record Identifier
- 9984094765302771
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