Journal article
A Comparison of Murine Smooth Muscle Cells Generated from Embryonic versus Induced Pluripotent Stem Cells
Stem cells and development, Vol.18(5), pp.741-748
06/01/2009
DOI: 10.1089/scd.2008.0179
PMCID: PMC2914231
PMID: 18795840
Abstract
Smooth muscle cell (SMC) differentiation and dedifferentiation play a critical role in the pathogenesis of cardiovascular diseases. The lack of a good and simple in vitro SMC differentiation system has hampered the progress of SMC field for years. The generation of such an in vitro system would be invaluable for exploring molecular mechanisms of SMC differentiation and dedifferentiation. Recently, the establishment of induced pluripotent stem (iPS) cells has offered a novel therapeutic strategy to generate patient-specific stem cell lines. Here we have investigated whether iPS cells are able to differentiate into SMCs in vitro. Mouse iPS cell (O9 and TT025) mono-layers were treated with 10(-5) mol/L all-trans retinoid acid (RA). After 8 days of RA treatment, we found that >40% of the O9 iPS cells expressed the SMC-markers including SM alpha-actin and SM myosin heavy chain. Also, we documented that iPS-derived SMCs acquired SMC functional characteristics including contraction and calcium influx in response to stimuli. Moreover, our results indicated that there were differences in SMC-specific gene expression patterns between SMCs derived from O9 and TT025 iPS as well as normal embryonic stem cells. These differences might be due to disparity in the current iPS technology. Taken together, our data have established a simple iPS-SMC system to generate SMCs in vitro, which has tremendous potential to generate individualized SMCs for vascular tissue engineering and personalized drug screening.
Details
- Title: Subtitle
- A Comparison of Murine Smooth Muscle Cells Generated from Embryonic versus Induced Pluripotent Stem Cells
- Creators
- Chang-Qing Xie - University of Michigan–Ann ArborHuarong Huang - University of Michigan–Ann ArborSheng Wei - University of IowaLong-Sheng Song - University of IowaJifeng Zhang - Samuel and Jean Frankel Cardiovascular CenterRaquel P. Ritchie - University of Michigan–Ann ArborLiangbiao Chen - Institute of Genetics and Developmental BiologyMing Zhang - Zhejiang UniversityY. Eugene Chen - Internal Medicine - Cardiology
- Resource Type
- Journal article
- Publication Details
- Stem cells and development, Vol.18(5), pp.741-748
- Publisher
- Mary Ann Liebert, Inc
- DOI
- 10.1089/scd.2008.0179
- PMID
- 18795840
- PMCID
- PMC2914231
- ISSN
- 1547-3287
- eISSN
- 1557-8534
- Number of pages
- 8
- Grant note
- 0840025N / American Heart Association HL68878; HL89544; HL75397; HL92421 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA R01HL090905; R01HL068878; R01HL075397; R01HL089544; R21HL092421 / NATIONAL HEART, LUNG, AND BLOOD INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI) 2007CB947800 / Ministry of Science and Technology of China; Ministry of Science and Technology, China
- Language
- English
- Date published
- 06/01/2009
- Academic Unit
- Cardiovascular Medicine; Fraternal Order of Eagles Diabetes Research Center; Biochemistry and Molecular Biology; Internal Medicine
- Record Identifier
- 9984288723202771
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