Journal article
A Comparison of the Genotyping Results Using DNA Obtained from Blood and Saliva
Psychiatric genetics, Vol.18(6), pp.275-281
12/2008
DOI: 10.1097/YPG.0b013e3283060f81
PMCID: PMC2648613
PMID: 19018232
Abstract
Traditionally, large scale genotyping projects have used DNA derived from whole blood or lymphoblast cell lines. But over the past several years, a number of investigators have begun to use DNA prepared from saliva for genotyping studies, particularly for use in behavioral genetic studies. However, the comparability of DNA from these two sources has not been rigorously analyzed by unbiased sources. In this communication, we compare the single nucleotide polymorphism (SNP) genotyping results from DNA derived from whole blood samples obtained from 474 subjects from the Iowa Adoption Studies with that saliva samples prepared from 555 members of the Strong African American Families project. We found that DNA prepared from whole blood performed significantly better than that prepared from saliva. Genotyping success was significantly associated with the concentration of human DNA in the saliva sample as determined by quantitative PCR, but not with the total amount of DNA as determined by UV spectroscopy. We conclude that investigators contemplating the choice of source materials of DNA for genotyping studies will need to balance the ease and economy of saliva based DNA collection methods with the higher yields and rates of genotyping calls associated with DNA prepared from whole blood.
Details
- Title: Subtitle
- A Comparison of the Genotyping Results Using DNA Obtained from Blood and Saliva
- Creators
- Robert A Philibert - Department of Child and Family Development and Center for Family Research, The University of GeorgiaOlga Zadorozhnyaya - Department of Child and Family Development and Center for Family Research, The University of GeorgiaSteven R. H Beach - Department of Child and Family Development and Center for Family Research, The University of GeorgiaGene H Brody - Department of Child and Family Development and Center for Family Research, The University of Georgia
- Resource Type
- Journal article
- Publication Details
- Psychiatric genetics, Vol.18(6), pp.275-281
- DOI
- 10.1097/YPG.0b013e3283060f81
- PMID
- 19018232
- PMCID
- PMC2648613
- NLM abbreviation
- Psychiatr Genet
- ISSN
- 0955-8829
- eISSN
- 1473-5873
- Language
- English
- Date published
- 12/2008
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Psychiatry; Iowa Neuroscience Institute
- Record Identifier
- 9984003901502771
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