Journal article
A Comprehensive Approach to Sample Preparation for Electron Microscopy and the Assessment of Mitochondrial Morphology in Tissue and Cultured Cells
Advanced biology, Vol.7(10), e2200202
10/2023
DOI: 10.1002/adbi.202200202
PMCID: PMC10615857
PMID: 37140138
Abstract
Mitochondria respond to metabolic demands of the cell and to incremental damage, in part, through dynamic structural changes that include fission (fragmentation), fusion (merging of distinct mitochondria), autophagic degradation (mitophagy), and biogenic interactions with the endoplasmic reticulum (ER). High resolution study of mitochondrial structural and functional relationships requires rapid preservation of specimens to reduce technical artifacts coupled with quantitative assessment of mitochondrial architecture. A practical approach for assessing mitochondrial fine structure using two dimensional and three dimensional high-resolution electron microscopy is presented, and a systematic approach to measure mitochondrial architecture, including volume, length, hyperbranching, cristae morphology, and the number and extent of interaction with the ER is described. These methods are used to assess mitochondrial architecture in cells and tissue with high energy demand, including skeletal muscle cells, mouse brain tissue, and Drosophila muscles. The accuracy of assessment is validated in cells and tissue with deletion of genes involved in mitochondrial dynamics.
Details
- Title: Subtitle
- A Comprehensive Approach to Sample Preparation for Electron Microscopy and the Assessment of Mitochondrial Morphology in Tissue and Cultured Cells
- Creators
- Antentor Hinton Jr - Univ Iowa, Carver Coll Med, Dept Internal Med, 200 Hawkins Dr, Iowa City, IA 52242 USAPrasanna Katti - National Heart Lung and Blood InstituteTrace A. Christensen - Mayo ClinicMargaret Mungai - University of IowaJianqiang Shao - University of IowaLiang Zhang - Mayo Clinic in FloridaSergey Trushin - Mayo ClinicAhmad Alghanem - Washington University in St. LouisAdam Jaspersen - Mayo ClinicRachel E. Geroux - Mayo ClinicKit Neikirk - University of Hawaii at HiloMichelle Biete - University of Hawaii at HiloEdgar Garza-Lopez - University of IowaBryanna Shao - Vanderbilt UniversityZer Vue - Vanderbilt UniversityLarry Vang - Vanderbilt UniversityHeather K. Beasley - Vanderbilt UniversityAndrea G. Marshall - Vanderbilt UniversityDominique Stephens - Vanderbilt UniversitySteven Damo - Fisk UniversityJessica Ponce - University of UtahChristopher K. E. Bleck - National Heart Lung and Blood InstituteInnes Hicsasmaz - University of IowaSandra A. Murray - University of PittsburghRanthony A. C. Edmonds - The Ohio State UniversityAndres Dajles - University of IowaYoung Do Koo - University of IowaSerif Bacevac - University of IowaJeffrey L. Salisbury - Mayo ClinicRenata O. Pereira - University of IowaBrian Glancy - National Heart Lung and Blood InstituteEugenia Trushina - Mayo ClinicE. Dale Abel - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Advanced biology, Vol.7(10), e2200202
- DOI
- 10.1002/adbi.202200202
- PMID
- 37140138
- PMCID
- PMC10615857
- NLM abbreviation
- Adv Biol (Weinh)
- ISSN
- 2701-0198
- eISSN
- 2701-0198
- Publisher
- Wiley
- Number of pages
- 20
- Grant note
- EES2112556; EES1817282; MCB1955975 / Burroughs Welcome Fund (BWF); Burroughs Wellcome Fund Vanderbilt Diabetes and Research Training Center for DRTC Alzheimer's Disease Pilot & Feasibility Program DK007563 / National Institute of Health (NIH) NIDDK T-32 Integrated Training in Engineering and Diabetes 1022355 / NSF MCB; National Science Foundation (NSF); NSF - Directorate for Biological Sciences (BIO) 1022376 / Burroughs Wellcome Fund Postdoctoral Enrichment Program; Burroughs Wellcome Fund T32~DK101003 / Burroughs Wellcome Fund/PDEP; Burroughs Wellcome Fund Burroughs Wellcome Fund Postdoctoral Enrichment Program; Burroughs Wellcome Fund 2022-253614; DK020593 / National Institutes of Health PRIDE Program; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA UNCF/Bristol-Myers Squibb (UNCF/BMS)- E.E. Just Postgraduate Fellowship in Life sciences Fellowship 1022355 / Multidisciplinary Training in Molecular Endocrinology 2011577I / UNCF/Bristol-Myers Squibb E.E. Just Faculty Fund NSF; National Science Foundation (NSF) R01HL108379; R01DK092065; R01NS107265; RO1AG062135; RO1AG59093; RO1AG072898; RF1AG55549-06; UG3/UH3~NS113776-2 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA 1022376 / Chan Zuckerberg Initiative DAF; Chan Zuckerberg Initiative (CZI) Multidisciplinary Training in Molecular Endocrinology
- Language
- English
- Electronic publication date
- 05/04/2023
- Date published
- 10/2023
- Academic Unit
- Fraternal Order of Eagles Diabetes Research Center; Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984420942202771
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