Journal article
A Conformational Change in Heparan Sulfate 3-O-Sulfotransferase-1 Is Induced by Binding to Heparan Sulfate
Biochemistry (Easton), Vol.43(16), pp.4680-4688
04/27/2004
DOI: 10.1021/bi0499112
PMID: 15096036
Abstract
The 3-O-sulfation of glucosamine by heparan sulfate 3-O-sulfotransferase-1 (3-OST-1) is a key modification step during the biosynthesis of anticoagulant heparan sulfate (HS). In this paper, we present evidence of a conformational change that occurs in 3-OST-1 upon binding to heparan sulfate. The intrinsic fluorescence of 3-OST-1 was increased in the presence of HS, suggesting a conformational change. This apparent conformational change was further investigated using differential chemical modification of 3-OST-1 to measure the solvent accessibility of the lysine residues. 3-OST-1 was treated with acetic anhydride in either the presence or absence of HS using both acetic anhydride and hexadeuterioacetic anhydride under nondenaturing and denaturing conditions, respectively. The relative reactivity of the lysine residues to acetylation and [2H] acetylation in the presence or absence of HS was analyzed by measuring the ratio of acetylated and deuterioacetylated peptides using matrix-assisted laser desorption ionization mass spectrometry. The solvent accessibilities of the lysine residues were altered differentially depending on their location. In particular, we observed a group of lysine residues in the C-terminus of 3-OST-1 that become more solvent accessible when 3-OST-1 binds to HS. This observation indicates that a conformational change could be occurring during substrate binding. A truncated mutant of 3-OST-1 that lacked this C-terminal region was expressed and found to exhibit a 200-fold reduction in sulfotransferase activity. The results from this study will contribute to our understanding of the interactions between 3-OSTs and HS.
Details
- Title: Subtitle
- A Conformational Change in Heparan Sulfate 3-O-Sulfotransferase-1 Is Induced by Binding to Heparan Sulfate
- Creators
- Suzanne C. Edavettal - National Institute of Environmental Health SciencesKevin Carrick - National Institute of Environmental Health SciencesRuchir R. Shah - National Institute of Environmental Health SciencesLars C. Pedersen - National Institute of Environmental Health SciencesAlexander Tropsha - National Institute of Environmental Health SciencesR. Marshall Pope - National Institute of Environmental Health SciencesJian Liu - National Institute of Environmental Health Sciences
- Resource Type
- Journal article
- Publication Details
- Biochemistry (Easton), Vol.43(16), pp.4680-4688
- Publisher
- American Chemical Society
- DOI
- 10.1021/bi0499112
- PMID
- 15096036
- ISSN
- 0006-2960
- eISSN
- 1520-4995
- Language
- English
- Date published
- 04/27/2004
- Academic Unit
- Medicine Administration
- Record Identifier
- 9984627209102771
Metrics
14 Record Views