Journal article
A Culture-Independent Analysis of the Microbiota of Female Interstitial Cystitis/Bladder Pain Syndrome Participants in the MAPP Research Network
Journal of clinical medicine, Vol.8(3), p.415
03/26/2019
DOI: 10.3390/jcm8030415
PMCID: PMC6462969
PMID: 30917614
Abstract
We surveyed urine microbiota of females diagnosed with interstitial cystitis/bladder pain syndrome (IC/BPS) and matched control participants enrolled in the National Institutes of Health (NIH) Multidisciplinary Approach to the Study of Chronic Pelvic Pain (MAPP) Research Network using the culture-independent methodology. Midstream urine specimens were analyzed with the Plex-ID molecular diagnostic platform that utilizes polymerase chain reaction-electrospray ionization-time-of-flight-mass spectrometry (PCR-ESI-TOF MS) to provide a comprehensive identification of bacterial and select fungal species. IC/BPS and control participants were evaluated for differences (presence, diversity, and abundance) in species and genus. Urine specimens obtained from 181 female IC/BPS and 182 female control participants detected a total of 92 species (41 genera). Mean (SD) species count was 2.49 (1.48) and 2.30 (1.28) among IC/BPS and control participants, respectively. Overall species composition did not significantly differ between IC/BPS and control participants at any level (p = 0.726 species level, p = 0.222 genus level). IC/BPS participants urine trended to an overabundance of Lactobacillus gasseri (p = 0.09) detected but had a lower prevalence of Corynebacterium compared with control participants (p = 0.002). The relative abundance data analysis mirrored the prevalence data differences with no significant differences in most species or genus abundance other than Lactobacillus gasseri and Corynebacterium (p = 0.08 and p = 0.001, respectively). No cause and/or effect conclusion can be drawn from this observation, but it suggests that a more comprehensive evaluation (vaginal, bowel, catheterized bladder and/or tissue-based specimens) of the lower urinary tract microbiota in IC/BPS patients is warranted.
Details
- Title: Subtitle
- A Culture-Independent Analysis of the Microbiota of Female Interstitial Cystitis/Bladder Pain Syndrome Participants in the MAPP Research Network
- Creators
- J. Curtis Nickel - Queen's UniversityAlisa J. Stephens-Shields - University of PennsylvaniaJ. Richard Landis - University of PennsylvaniaChris Mullins - National Institute of Diabetes and Digestive and Kidney DiseasesAdrie van Bokhoven - University of Colorado Anschutz Medical CampusM. Scott Lucia - University of Colorado Anschutz Medical CampusJeffrey P. Henderson - Washington University in St. LouisBhaswati Sen - Drexel UniversityJaroslaw E. Krol - Drexel Univ, Coll Med, Dept Microbiol, Philadelphia, PA 19102 USAGarth D. Ehrlich - Drexel UniversityJ. Quentin Clemens - MAPP Network Execut Comm, Indianapolis, IN USAPhilip Hanno - MAPP Network Execut Comm, Indianapolis, IN USAZiya Kirkali - MAPP Network Execut Comm, Indianapolis, IN USAJohn W. Kusek - MAPP Network Execut Comm, Indianapolis, IN USAMichel A. Pontari - MAPP Network Execut Comm, Indianapolis, IN USADavid J. Klumpp - Northwestern Univ, Discovery Site, Evanston, IL 60208 USAAnthony J. Schaeffer - Northwestern Univ, Discovery Site, Evanston, IL 60208 USAApkar (Vania) Apkarian - Northwestern Univ, Discovery Site, Evanston, IL 60208 USADavid Cella - Northwestern Univ, Discovery Site, Evanston, IL 60208 USAMelissa A. Farmer - Northwestern Univ, Discovery Site, Evanston, IL 60208 USAColleen Fitzgerald - Northwestern Univ, Discovery Site, Evanston, IL 60208 USARichard Gershon - Northwestern Univ, Discovery Site, Evanston, IL 60208 USAJames W. Griffith - Northwestern UniversityCharles J. Heckman - Northwestern Univ, Discovery Site, Evanston, IL 60208 USAMingchen Jiang - Northwestern Univ, Discovery Site, Evanston, IL 60208 USALaurie Keefer - Northwestern UniversityDarlene S. Marko - Northwestern Univ, Discovery Site, Evanston, IL 60208 USAJean Michniewicz - Northwestern Univ, Discovery Site, Evanston, IL 60208 USATodd Parrish - Northwestern Univ, Discovery Site, Evanston, IL 60208 USAFrank Tu - Northwestern Univ, Discovery Site, Evanston, IL 60208 USAEmeran A. Mayer - Univ Calif Los Angeles, Discovery Site, Los Angeles, CA USALarissa Rodriguez - Univ Calif Los Angeles, Discovery Site, Los Angeles, CA USAJeffry Alger - Univ Calif Los Angeles, Discovery Site, Los Angeles, CA USACody P. Ashe-McNalley - Univ Calif Los Angeles, Discovery Site, Los Angeles, CA USABen Ellingson - Univ Calif Los Angeles, Discovery Site, Los Angeles, CA USANuwanthi Heendeniya - Univ Calif Los Angeles, Discovery Site, Los Angeles, CA USALisa Kilpatrick - Univ Calif Los Angeles, Discovery Site, Los Angeles, CA USAJason Kutch - Univ Calif Los Angeles, Discovery Site, Los Angeles, CA USAJennifer S. Labus - Univ Calif Los Angeles, Discovery Site, Los Angeles, CA USABruce D. Naliboff - Univ Calif Los Angeles, Discovery Site, Los Angeles, CA USAFornessa Randal - Univ Calif Los Angeles, Discovery Site, Los Angeles, CA USASuzanne R. Smith - Univ Calif Los Angeles, Discovery Site, Los Angeles, CA USAKarl J. Kreder - University of IowaCatherine S. Bradley - University of IowaMary Eno - Univ Iowa, Discovery Site, Iowa City, IA 52242 USAKris Greiner - Univ Iowa, Discovery Site, Iowa City, IA 52242 USAYi Luo - University of IowaSusan K. Lutgendorf - University of IowaMichael A. O'Donnell - University of IowaBarbara Ziegler - Univ Iowa, Discovery Site, Iowa City, IA 52242 USADaniel J. Clauw - Univ Michigan, Discovery Site, Ann Arbor, MI 48109 USASuzie As-Sanie - Univ Michigan, Discovery Site, Ann Arbor, MI 48109 USASandra Berry - Univ Michigan, Discovery Site, Ann Arbor, MI 48109 USAMegan E. Halvorson - Univ Michigan, Discovery Site, Ann Arbor, MI 48109 USARichard Harris - Univ Michigan, Discovery Site, Ann Arbor, MI 48109 USASteve Harte - Univ Michigan, Discovery Site, Ann Arbor, MI 48109 USAEric Ichesco - Univ Michigan, Discovery Site, Ann Arbor, MI 48109 USAAnn Oldendorf - Univ Michigan, Discovery Site, Ann Arbor, MI 48109 USAKatherine A. Scott - Univ Michigan, Discovery Site, Ann Arbor, MI 48109 USADavid A. Williams - Univ Michigan, Discovery Site, Ann Arbor, MI 48109 USADedra Buchwald - Univ Washington, Discovery Site, Seattle, WA 98195 USANiloofar Afari - Univ Washington, Discovery Site, Seattle, WA 98195 USAJohn Krieger - Univ Washington, Discovery Site, Seattle, WA 98195 USAJane Miller - University of WashingtonStephanie Richey - Univ Washington, Discovery Site, Seattle, WA 98195 USASusan O. Ross - Univ Washington, Discovery Site, Seattle, WA 98195 USARoberta Spiro - Univ Washington, Discovery Site, Seattle, WA 98195 USAT. J. Sundsvold - Univ Washington, Discovery Site, Seattle, WA 98195 USAEric Strachan - Univ Washington, Discovery Site, Seattle, WA 98195 USAClaire C. Yang - Univ Washington, Discovery Site, Seattle, WA 98195 USAGerald L. Andriole - Washington Univ, Discovery Site, St Louis, MO 63130 USAH. Henry Lai - Washington Univ, Discovery Site, St Louis, MO 63130 USARebecca L. Bristol - Washington Univ, Discovery Site, St Louis, MO 63130 USAGraham Colditz - Washington Univ, Discovery Site, St Louis, MO 63130 USAGeorg Deutsch - Washington Univ, Discovery Site, St Louis, MO 63130 USAVivien C. Gardner - Washington Univ, Discovery Site, St Louis, MO 63130 USARobert W. Gereau - Washington Univ, Discovery Site, St Louis, MO 63130 USABarry A. Hong - Washington Univ, Discovery Site, St Louis, MO 63130 USAThomas M. Hooton - Washington Univ, Discovery Site, St Louis, MO 63130 USATimothy J. Ness - Washington Univ, Discovery Site, St Louis, MO 63130 USACarol S. North - Washington Univ, Discovery Site, St Louis, MO 63130 USATheresa M. Spitznagle - Washington Univ, Discovery Site, St Louis, MO 63130 USASiobhan Sutcliffe - Washington Univ, Discovery Site, St Louis, MO 63130 USATed Barrell - Univ Penn, DCC, Philadelphia, PA 19104 USATheressa Creighton - Univ Penn, DCC, Philadelphia, PA 19104 USALaura Fluharty - Univ Penn, DCC, Philadelphia, PA 19104 USAXlaoling Hou - Univ Penn, DCC, Philadelphia, PA 19104 USANancy Robinson - Univ Penn, DCC, Philadelphia, PA 19104 USAAlisa Stephens - Univ Penn, DCC, Philadelphia, PA 19104 USAYanli Wang - Univ Penn, DCC, Philadelphia, PA 19104 USAAndrea A. Osypuk - Univ Colorado Denver, TATC, Denver, CO USARobert Dayton - Univ Colorado Denver, TATC, Denver, CO USAKaren R. Jonscher - Univ Colorado Denver, TATC, Denver, CO USAHolly T. Sullivan - Univ Colorado Denver, TATC, Denver, CO USAR. Storey Wilson - Univ Colorado Denver, TATC, Denver, CO USAMarsha A. Moses - Harvard Med Sch, Boston Childrens Hosp, Boston, MA 02115 USAAndrew C. Briscoe - Harvard Med Sch, Boston Childrens Hosp, Boston, MA 02115 USADavid Briscoe - Harvard Med Sch, Boston Childrens Hosp, Boston, MA 02115 USAAdam Curatolo - Harvard Med Sch, Boston Childrens Hosp, Boston, MA 02115 USAJohn Froehlich - Harvard Med Sch, Boston Childrens Hosp, Boston, MA 02115 USARichard S. Lee - Harvard Med Sch, Boston Childrens Hosp, Boston, MA 02115 USAMonisha Sachdev - Harvard Med Sch, Boston Childrens Hosp, Boston, MA 02115 USAKeith R. Solomon - Harvard Med Sch, Boston Childrens Hosp, Boston, MA 02115 USAHanno Steen - Harvard Medical SchoolSean Mackey - Stanford UniversityEpifanio Bagarinao - Stanford Univ, Stanford, CA 94305 USALauren C. Foster - Stanford Univ, Stanford, CA 94305 USAEmily Hubbard - Stanford Univ, Stanford, CA 94305 USAKevin A. Johnson - Stanford UniversityKatherine T. Martucci - Stanford Univ, Stanford, CA 94305 USARebecca L. McCue - Stanford Univ, Stanford, CA 94305 USARachel R. Moericke - Stanford Univ, Stanford, CA 94305 USAAneesha Nilakantan - Stanford Univ, Stanford, CA 94305 USANoorulain Noor - Stanford Univ, Stanford, CA 94305 USATamara G. Bavendam - NIDDK, NIH, Bethesda, MD 26506 USAMultidisciplinary Approach to the Study of Chronic Pelvic Pain (MAPP) Research Network
- Resource Type
- Journal article
- Publication Details
- Journal of clinical medicine, Vol.8(3), p.415
- DOI
- 10.3390/jcm8030415
- PMID
- 30917614
- PMCID
- PMC6462969
- NLM abbreviation
- J Clin Med
- ISSN
- 2077-0383
- eISSN
- 2077-0383
- Publisher
- Mdpi
- Number of pages
- 14
- Grant note
- DK82370; DK82342; DK82315; DK82344; DK82325; DK82345; DK82333; DK82316 / National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health (NIH) MAPP Network Awards U01DK082370 / NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK)
- Language
- English
- Date published
- 03/26/2019
- Academic Unit
- Epidemiology; Psychological and Brain Sciences; Iowa Neuroscience Institute; Obstetrics and Gynecology; Urology
- Record Identifier
- 9984316904502771
Metrics
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