Journal article
A DNA transposon-based approach to validate oncogenic mutations in the mouse
Proceedings of the National Academy of Sciences - PNAS, Vol.105(50), pp.19904-19909
12/16/2008
DOI: 10.1073/pnas.0807785105
PMCID: PMC2597691
PMID: 19064922
Abstract
Large-scale cancer genome projects will soon be able to sequence many cancer genomes to comprehensively identify genetic changes in human cancer. Genome-wide association studies have also identified putative cancer associated loci. Functional validation of these genetic mutations
in vivo
is becoming a challenge. We describe here a DNA transposon-based platform that permits us to explore the oncogenic potential of genetic mutations in the mouse. Briefly, promoter-less human cancer gene cDNAs were first cloned into
Sleeping Beauty
(
SB
) transposons. DNA transposition in the mouse that carried both the transposons and the SB transposase made it possible for the cDNAs to be expressed from an appropriate endogenous genomic locus and in the relevant cell types for tumor development. Consequently, these mice developed a broad spectrum of tumors at very early postnatal stages. This technology thus complements the large-scale cancer genome projects.
Details
- Title: Subtitle
- A DNA transposon-based approach to validate oncogenic mutations in the mouse
- Creators
- Qin Su - Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, EnglandHaydn M Prosser - Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, EnglandLia S Campos - Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, EnglandMariaestela Ortiz - Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, EnglandTakuro Nakamura - The Cancer Institute, Japanese Foundation for Cancer Research, 3-10-6 Ariake, Koto-ku, Tokyo 135-8550, JapanMadhuri Warren - Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, EnglandAdam J Dupuy - Department of Anatomy and Cell Biology, University of Iowa, 100 Bowen Science Building, 51 Newton Road, Iowa City, IA 52242-1109; andNancy A Jenkins - Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673Neal G Copeland - Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673Allan Bradley - Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, EnglandPentao Liu - Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, England
- Resource Type
- Journal article
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.105(50), pp.19904-19909
- DOI
- 10.1073/pnas.0807785105
- PMID
- 19064922
- PMCID
- PMC2597691
- NLM abbreviation
- Proc Natl Acad Sci U S A
- ISSN
- 0027-8424
- eISSN
- 1091-6490
- Publisher
- National Academy of Sciences
- Language
- English
- Date published
- 12/16/2008
- Academic Unit
- Anatomy and Cell Biology; Pathology
- Record Identifier
- 9984025365802771
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