Journal article
A Drosophila CREB/ATF transcriptional activator binds to both fat body- and liver-specific regulatory elements
Genes & development, Vol.6(3), pp.466-480
1992
DOI: 10.1101/gad.6.3.466
PMID: 1532159
Abstract
We have identified a Drosophila transcription factor that binds to fat body-specific enhancers of alcohol dehydrogenase (Adh) and yolk protein genes. DNA sequence analysis of cDNA clones encoding this protein, box B-binding factor-2 (BBF-2), indicates that it is a member of the CREB/ATF family of transcriptional regulatory proteins. A number of observations suggest that BBF-2 is involved in fat body-specific expression: Mutations that disrupt BBF-2 binding to two different Adh fat body enhancers in vitro decrease the activity of these enhancers in transgenic flies. BBF-2 mRNA is present in all cell types examined, and the protein is present in cells that express ADH. Finally, BBF-2 is a transcriptional activator in Drosophila tissue culture cells. Remarkably, BBF-2 also binds specifically to regulatory elements required for liver-specific expression of the human Adh and rat tyrosine aminotransferase genes. Thus, BBF-2 and the DNA sequence to which it binds may be important components of a tissue-specific regulatory mechanism conserved between Drosophila and man.
Details
- Title: Subtitle
- A Drosophila CREB/ATF transcriptional activator binds to both fat body- and liver-specific regulatory elements
- Creators
- T ABEL - Harvard univ., dep. biochemistry molecular biology, Cambridge MA 02138, United StatesR BHATT - Harvard univ., dep. biochemistry molecular biology, Cambridge MA 02138, United StatesT MANIATIS - Harvard univ., dep. biochemistry molecular biology, Cambridge MA 02138, United States
- Resource Type
- Journal article
- Publication Details
- Genes & development, Vol.6(3), pp.466-480
- Publisher
- Cold Spring Harbor Laboratory; Cold Spring Harbor, NY
- DOI
- 10.1101/gad.6.3.466
- PMID
- 1532159
- ISSN
- 0890-9369
- eISSN
- 1549-5477
- Language
- English
- Date published
- 1992
- Academic Unit
- Molecular Physiology and Biophysics; Psychiatry; Psychological and Brain Sciences; Iowa Neuroscience Institute; Neuroscience and Pharmacology; Biochemistry and Molecular Biology
- Record Identifier
- 9984065826702771
Metrics
25 Record Views