A Drosophila GATA family member that binds to Adh regulatory sequences is expressed in the developing fat body

T ABEL; A. M MICHELSON; T MANIATIS

doi:10.1242/dev.119.3.623

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A Drosophila GATA family member that binds to Adh regulatory sequences is expressed in the developing fat body

Journal article

Peer reviewed

A Drosophila GATA family member that binds to Adh regulatory sequences is expressed in the developing fat body

T ABEL, A. M MICHELSON and T MANIATIS

Development (Cambridge), Vol.119(3), pp.623-633

1993

DOI: 10.1242/dev.119.3.623

PMID: 8187633

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Abstract

We have identified a Drosophila transcription factor that binds a sequence element found in the larval promoters of all known alcohol dehydrogenase (Adh) genes. DNA sequence analysis of cDNA clones encoding this protein, box A-binding factor (ABF), reveals that it is a member of the GATA family of transcriptional regulatory factors. ABF-binding sites within the D. mulleri and D. melanogaster larval Adh promoters function as positive regulatory elements and in cotransfection experiments, ABF functions as a transcriptional activator. In further support of a role for ABF in the regulation of Adh expression, ABF mRNA is expressed in the embryonic fat body, a tissue that contains high levels of Adh mRNA. Our studies demonstrate that the fat body develops from segmentally repeated clusters of mesodermal cells, which later expand and coalesce to form the mature fat body. These observations establish ABF as the earliest known fat body precursor marker in the Drosophila embryo. Together with the established role of GATA factors during mammalian development, these results suggest that ABF may play a key role in the organogenesis of the fat body.

Fundamental and applied biological sciences. Psychology

Biochemistry. Physiology. Immunology

Biological and medical sciences

Physiology. Development

Invertebrates

Insecta

Details

Title: Subtitle: A Drosophila GATA family member that binds to Adh regulatory sequences is expressed in the developing fat body
Creators: T ABEL - Harvard univ., dep. biochemistry molecular biology, Cambridge MA 02138, United States
A. M MICHELSON - Harvard univ., dep. biochemistry molecular biology, Cambridge MA 02138, United States
T MANIATIS - Harvard univ., dep. biochemistry molecular biology, Cambridge MA 02138, United States
Resource Type: Journal article
Publication Details: Development (Cambridge), Vol.119(3), pp.623-633
DOI: 10.1242/dev.119.3.623
PMID: 8187633
NLM abbreviation: Development
ISSN: 0950-1991
eISSN: 1477-9129
Publisher: Company of Biologists; Cambridge
Language: English
Date published: 1993
Academic Unit: Molecular Physiology and Biophysics; Psychiatry; Psychological and Brain Sciences; Iowa Neuroscience Institute; Neuroscience and Pharmacology; Biochemistry and Molecular Biology
Record Identifier: 9984071746702771

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