Journal article
A Flow Imaging Microscopy–Based Method Using Mass-to-Volume Ratio to Derive the Porosity of PLGA Microparticles
Journal of pharmaceutical sciences, Vol.106(11), pp.3378-3384
11/2017
DOI: 10.1016/j.xphs.2017.07.015
PMID: 28755925
Abstract
The release of drugs from poly(lactic-co-glycolic acid) (PLGA) microparticles depends to a large extent on the porosity of the particles. Therefore, porosity determination of PLGA microparticles is extremely important during pharmaceutical product development. Currently, mercury intrusion porosimetry (MIP) is widely used despite its disadvantages, such as the need for a large amount of sample (several hundreds of milligrams) and residual toxic waste. Here, we present a method based on the estimation of the volume of a known mass (a few milligrams) of particles using micro-flow imaging (MFI) to determine microparticle batch porosity. Factors that are critical for the accuracy of this method (i.e., density of the suspending fluid, particle concentration, and postsample rinsing) were identified and measures were taken to minimize potential errors. The validity of the optimized method was confirmed by using nonporous polymethylmethacrylate microparticles. Finally, the method was employed for the analysis of 7 different PLGA microparticle batches with various porosities (4.0%-51.9%) and drug loadings (0%-38%). Obtained porosity values were in excellent agreement with the MIP-derived porosities. Altogether, the developed MFI-based method is a valuable tool for deriving the total volume of a known mass of PLGA particles and therewith their porosity.
Details
- Title: Subtitle
- A Flow Imaging Microscopy–Based Method Using Mass-to-Volume Ratio to Derive the Porosity of PLGA Microparticles
- Creators
- Ahmad S. Sediq - Centre for Human Drug ResearchStephan K.D. Waasdorp - Centre for Human Drug ResearchM. Reza Nejadnik - Centre for Human Drug ResearchMiranda M.C. van Beers - Centre for Human Drug ResearchJelte Meulenaar - Dr. Reddy's LaboratoriesRuud Verrijk - Dr. Reddy's LaboratoriesWim Jiskoot - Centre for Human Drug Research
- Resource Type
- Journal article
- Publication Details
- Journal of pharmaceutical sciences, Vol.106(11), pp.3378-3384
- DOI
- 10.1016/j.xphs.2017.07.015
- PMID
- 28755925
- NLM abbreviation
- J Pharm Sci
- ISSN
- 0022-3549
- eISSN
- 1520-6017
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 11/2017
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics
- Record Identifier
- 9984420837302771
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