Journal article
A Fluorescent Sensor and Gel Stain for Detection of Pyrophosphorylated Proteins
ACS chemical biology, Vol.10(9), pp.1958-1963
09/18/2015
DOI: 10.1021/acschembio.5b00256
PMID: 26061479
Abstract
The design and synthesis of a fluorescent sensor of diphosphate esters along with its application for in-gel detection is described. Dinuclear zinc complex 1 selectively binds diphosphate esters in the presence of various other functional groups, including monophosphate esters. Complex 1 also constitutes a competent stain for visualization of pyrophosphorylated proteins in polyacrylamide gels. This reagent will facilitate the validation and exploration of protein pyrophosphorylation.
Details
- Title: Subtitle
- A Fluorescent Sensor and Gel Stain for Detection of Pyrophosphorylated Proteins
- Creators
- Florence J Williams - Department of Chemistry, Princeton University , Washington Road, Princeton, New Jersey 08544, United StatesDorothea Fiedler - Department of Chemistry, Princeton University , Washington Road, Princeton, New Jersey 08544, United States
- Resource Type
- Journal article
- Publication Details
- ACS chemical biology, Vol.10(9), pp.1958-1963
- Publisher
- United States
- DOI
- 10.1021/acschembio.5b00256
- PMID
- 26061479
- ISSN
- 1554-8929
- eISSN
- 1554-8937
- Grant note
- DP2 CA186753 / NCI NIH HHS
- Language
- English
- Date published
- 09/18/2015
- Academic Unit
- Iowa Neuroscience Institute; Chemistry
- Record Identifier
- 9984065467202771
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