A General Strategy for Extrusion Bioprinting of Bio-Macromolecular Bioinks through Alginate-Templated Dual-Stage Crosslinking

Kai Zhu; Nan Chen; Xiao Liu; Xuan Mu; Weijia Zhang; Chunsheng Wang; Yu Shrike Zhang

doi:10.1002/mabi.201800127

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A General Strategy for Extrusion Bioprinting of Bio-Macromolecular Bioinks through Alginate-Templated Dual-Stage Crosslinking

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A General Strategy for Extrusion Bioprinting of Bio-Macromolecular Bioinks through Alginate-Templated Dual-Stage Crosslinking

Kai Zhu, Nan Chen, Xiao Liu, Xuan Mu, Weijia Zhang, Chunsheng Wang and Yu Shrike Zhang

Macromolecular bioscience, Vol.18(9), pp.e1800127-e1800127

06/25/2018

DOI: 10.1002/mabi.201800127

PMCID: PMC6467480

PMID: 29943499

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Abstract

The recently developed 3D bioprinting technology has greatly improved the ability to generate biomimetic tissues that are structurally and functionally relevant to their human counterparts. The selection of proper biomaterials as the bioinks is a key step toward successful bioprinting. For example, viscosity of a bioink is an important rheological parameter to determine the flexibility in deposition of free-standing structures and the maintenance of architectural integrity following bioprinting. This requirement, however, has greatly limited the selection of bioinks, especially for those naturally derived due to their commonly low mechanical properties. Here the generalization of a mechanism for extrusion bioprinting of bio-macromolecular components, mainly focusing on collagen and its derivatives including gelatin and gelatin methacryloyl, is reported. Specifically, a templating strategy is adopted using a composite bioink containing both the desired bio-macromolecular component and a polysaccharide alginate. The physically crosslinkable alginate component serves as the temporal structural support to stabilize the shape of the construct during bioprinting; upon subsequent chemical or physical crosslinking of the bio-macromolecular component, alginate can be selectively removed to leave only the desired biomacromolecule. It is anticipated that this strategy is general, and can be readily expanded for use of a wide variety of other bio-macromolecular bioinks.

alginate

bio-macromolecule

bioink

extrusion bioprinting

microfluidic bioprinting

Details

Title: Subtitle: A General Strategy for Extrusion Bioprinting of Bio-Macromolecular Bioinks through Alginate-Templated Dual-Stage Crosslinking
Creators: Kai Zhu - Fudan University
Nan Chen - Fudan University
Xiao Liu - Beihang University
Xuan Mu - Brigham and Women's Hospital
Weijia Zhang - Fudan University
Chunsheng Wang - Fudan University
Yu Shrike Zhang - Brigham and Women's Hospital
Resource Type: Journal article
Publication Details: Macromolecular bioscience, Vol.18(9), pp.e1800127-e1800127
DOI: 10.1002/mabi.201800127
PMID: 29943499
PMCID: PMC6467480
NLM abbreviation: Macromol Biosci
ISSN: 1616-5187
eISSN: 1616-5195
Grant note: DOI: 10.13039/100000054, name: National Cancer Institute; DOI: 10.13039/100000002, name: National Institutes of Health, award: K99CA201603; DOI: 10.13039/501100003399, name: Science and Technology Commission of Shanghai Municipality, award: 17JC 1400200; DOI: 10.13039/501100001809, name: National Natural Science Foundation of China, award: 81771971
Language: English
Date published: 06/25/2018
Academic Unit: Roy J. Carver Department of Biomedical Engineering
Record Identifier: 9984276452702771

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