Journal article
A Guide to Understanding "State-of-the-Art" Basic Research Techniques in Anesthesiology
Anesthesia and analgesia, Vol.131(2), pp.450-463
08/01/2020
DOI: 10.1213/ANE.0000000000004801
PMCID: PMC9020655
PMID: 32371742
Abstract
Perioperative medicine is changing from a "protocol-based" approach to a progressively personalized care model. New molecular techniques and comprehensive perioperative medical records allow for detection of patient-specific phenotypes that may better explain, or even predict, a patient's response to perioperative stress and anesthetic care. Basic science technology has significantly evolved in recent years with the advent of powerful approaches that have translational relevance. It is incumbent on us as a primarily clinical specialty to have an in-depth understanding of rapidly evolving underlying basic science techniques to incorporate such approaches into our own research, critically interpret the literature, and improve future anesthesia patient care. This review focuses on 3 important and most likely practice-changing basic science techniques: next-generation sequencing (NGS), clustered regularly interspaced short palindromic repeat (CRISPR) modulations, and inducible pluripotent stem cells (iPSCs). Each technique will be described, potential advantages and limitations discussed, open questions and challenges addressed, and future developments outlined. We hope to provide insight for practicing physicians when confronted with basic science articles and encourage investigators to apply "state-of-the-art" technology to their future experiments.
Details
- Title: Subtitle
- A Guide to Understanding "State-of-the-Art" Basic Research Techniques in Anesthesiology
- Creators
- Detlef Obal - Stanford UniversityShaogen Wu - Stanford UniversityAndrew McKinstry-Wu - University of PennsylvaniaVivianne L. Tawfik - Stanford University
- Resource Type
- Journal article
- Publication Details
- Anesthesia and analgesia, Vol.131(2), pp.450-463
- DOI
- 10.1213/ANE.0000000000004801
- PMID
- 32371742
- PMCID
- PMC9020655
- NLM abbreviation
- Anesth Analg
- ISSN
- 0003-2999
- eISSN
- 1526-7598
- Publisher
- Lippincott Williams & Wilkins
- Number of pages
- 14
- Grant note
- Rita Allen Foundation Award in Pain Seed Grant of the Stanford CV Institute K08NS094547; K08GM123317 / National Institutes of Health (NIH); United States Department of Health & Human Services; National Institutes of Health (NIH) - USA Transdisciplinary Initiatives Program (TIP) of the Stanford Maternal & Child Health Research Institute
- Language
- English
- Date published
- 08/01/2020
- Academic Unit
- Anesthesia
- Record Identifier
- 9984696860202771
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