Journal article
A Human RNA Polymerase II Transcription Termination Factor Is a SWI2/SNF2 Family Member
The Journal of biological chemistry, Vol.273(40), pp.25541-25544
10/02/1998
DOI: 10.1074/jbc.273.40.25541
PMID: 9748214
Abstract
We obtained protein sequence information from Drosophila factor 2, an ATP-dependent RNA polymerase II transcription termination factor, and discovered that it was identical to a SWI2/SNF2 family member called lodestar. Portions of putative human and Caenorhabditis elegans homologues were found in the sequence data bases and a complete cDNA for the human factor was generated using polymerase chain reaction techniques. Recombinant human factor 2 was produced in a baculovirus expression system, purified, and characterized. Similar to the authentic Drosophila factor, the human factor displayed a strong double-stranded DNA-dependent ATPase activity that was inhibited by single-stranded DNA and exhibited RNA polymerase II termination activity. Both factors were able to work on elongation complexes from either species. We discuss the mechanism of termination by factor 2 and the implications for the role of factor 2 in cellular activities.
Details
- Title: Subtitle
- A Human RNA Polymerase II Transcription Termination Factor Is a SWI2/SNF2 Family Member
- Creators
- Mingyi LiuZhi XieDavid H Price
- Resource Type
- Journal article
- Publication Details
- The Journal of biological chemistry, Vol.273(40), pp.25541-25544
- DOI
- 10.1074/jbc.273.40.25541
- PMID
- 9748214
- NLM abbreviation
- J Biol Chem
- ISSN
- 0021-9258
- eISSN
- 1083-351X
- Language
- English
- Date published
- 10/02/1998
- Academic Unit
- Biochemistry and Molecular Biology
- Record Identifier
- 9984025398302771
Metrics
24 Record Views