Journal article
A Large, Antigenically Conserved Protein On The Surface Of Moraxella Catarrhalis Is A Target For Protective Antibodies
The Journal of infectious diseases, Vol.170(4), pp.867-872
10/1994
DOI: 10.1093/infdis/170.4.867
PMID: 7523537
Abstract
A monoclonal antibody (MAb) to Moraxella catarrhalis 035E bound to a surface-exposed epitope of a proteinaceous antigen of this organism. The antigen, designated Usp.A, was present in every strain of the pathogen tested in a colony blot RIA. UspA had a molecular mass on SDS-PAGE that varied between 300 and 400 kDa, depending on the individual M. catarrhalis strain. Passive immunization of mice with the UspA-reactive Mab enhanced pulmonary clearance of M. catarrhalis. Use ofthis Mab to screen aM. catarrhalis genomic DNA library permitted identification of a recombinant bacteriophage expressing the M. catarrhalis UspA protein. The recombinant UspA protein was used in Western blot analysis with sera from patients with M. catarrhalis pneumonia. Convalescent-phase sera but not acute-phase sera from these patients contained antibodies to this M. catarrhalis surface protein, indicating that M. catarrhalis strains growing in vivo express this molecule.
Details
- Title: Subtitle
- A Large, Antigenically Conserved Protein On The Surface Of Moraxella Catarrhalis Is A Target For Protective Antibodies
- Creators
- Merja E Helminen - Departments of Microbiology and Pediatrics, University of Texas Southwestern Medical CenterDallasIsobel Maciver - Departments of Microbiology and Pediatrics, University of Texas Southwestern Medical CenterDallasJo L Latimer - Departments of Microbiology and Pediatrics, University of Texas Southwestern Medical CenterDallasJulia Klesney-Tait - Departments of Microbiology and Pediatrics, University of Texas Southwestern Medical CenterDallasLeslie D Cope - Departments of Microbiology and Pediatrics, University of Texas Southwestern Medical CenterDallasMaria Paris - Departments of Microbiology and Pediatrics, University of Texas Southwestern Medical CenterDallasGeorge H McCracken - Departments of Microbiology and Pediatrics, University of Texas Southwestern Medical CenterDallasEric J Hansen - Departments of Microbiology and Pediatrics, University of Texas Southwestern Medical CenterDallas
- Resource Type
- Journal article
- Publication Details
- The Journal of infectious diseases, Vol.170(4), pp.867-872
- Publisher
- The University of Chicago Press
- DOI
- 10.1093/infdis/170.4.867
- PMID
- 7523537
- ISSN
- 0022-1899
- eISSN
- 1537-6613
- Language
- English
- Date published
- 10/1994
- Academic Unit
- Pulmonary, Critical Care, and Occupational Medicine; Internal Medicine
- Record Identifier
- 9984094711002771
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