Journal article
A Minimally Invasive Method for Generating a Syngeneic Orthotopic Mouse Model of Lung Cancer
Journal of visualized experiments, Vol.222(222), e68676
08/19/2025
DOI: 10.3791/68676
PMID: 40920639
Abstract
Non-small cell lung cancer (NSCLC) continues to be the number one cause of cancer-related death for both women and men worldwide. More information needs to be gathered to understand the interactions between cancer cells, the immune system, the microenvironment within each tumor, and the host tissue to develop more effective treatment modalities. Reported here is a simple, repeatable method for inducing cancer within the mouse lung, allowing for the monitoring of tumor growth from early to late-stage disease. The surgical procedure involves a small superficial incision in the skin and an injection of cancer cells through the intercostal muscles into the lung. In vivo imaging systems (IVIS) in combination with cells engineered to express luciferase provide a convenient way of evaluating tumor growth and disease progression over the course of a study. This technique is easily transferable across various models of lung cancer and can be used to test the feasibility and efficacy of novel therapeutics in a preclinical model of lung cancer that mimics the natural tumor microenvironment. This broadens the relevance of both efficacy and safety/toxicity studies using standard-of-care treatments, including surgical resection, radiation, and immunotherapies in conjunction with experimental therapeutics.
Details
- Title: Subtitle
- A Minimally Invasive Method for Generating a Syngeneic Orthotopic Mouse Model of Lung Cancer
- Creators
- Danielle J. Foster - University of IowaSunny C. Huang - University of Iowa, Radiation OncologyJennifer A. Petsche - University of IowaCharles C. Searby - University of IowaTerry G. Beltz - University of IowaVal C. Sheffield - University of IowaCalvin S. Carter - University of Iowa, Iowa Neuroscience Institute
- Resource Type
- Journal article
- Publication Details
- Journal of visualized experiments, Vol.222(222), e68676
- DOI
- 10.3791/68676
- PMID
- 40920639
- NLM abbreviation
- J Vis Exp
- ISSN
- 1940-087X
- eISSN
- 1940-087X
- Publisher
- JOURNAL OF VISUALIZED EXPERIMENTS
- Grant note
- Department of Radiology at the University of IowaNational Institutes of Health grants: NCI R21CA256301 Free Radical Radiation Biology Program: NCI T32CA078586 IVIS Lumina S5 Imaging System purchased: 1S10OD026835
We would like to thank members of the Carter and Sheffield labs, Susan Walsh, Bryan Allen, and Kranti Mapuskar for valuable discussions. We would also like to thank Samantha Eckley, senior clinical veterinarian in the Office of Animal Resources, for her guidance and advice. We would like to acknowledge the use of the IVIS Lumina S5 Imaging System at the Small Animal Imaging Core Facility, a core resource supported by the Department of Radiology at the University of Iowa. Graphical illustrations from figures generated using Biorender (https://biorender.com ) . This work was supported by National Institutes of Health grants NCI R21CA256301 (C.S.C., V.C.S.) , Free Radical Radiation Biology Program NCI T32CA078586 (S.C.H.) , IVIS Lumina S5 Imaging System purchased on 1S10OD026835.
- Language
- English
- Date published
- 08/19/2025
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Medical Genetics and Genomics; Radiation Oncology; Neuroscience and Pharmacology; Ophthalmology and Visual Sciences
- Record Identifier
- 9984948111602771
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