Journal article
A Moderate Blast Exposure Results in Dysregulated Gene Network Activity Related to Cell Death, Survival, Structure, and Metabolism
Frontiers in neurology, Vol.11, pp.91-91
02/26/2020
DOI: 10.3389/fneur.2020.00091
PMCID: PMC7054450
PMID: 32174881
Abstract
Blast exposure is common in military personnel during training and combat operations, yet biological mechanisms related to cell survival and function that coordinate recovery remain poorly understood. This study explored how moderate blast exposure influences gene expression; specifically, gene-network changes following moderate blast exposure. On day 1 (baseline) of a 10-day military training program, blood samples were drawn, and health and demographic information collected. Helmets equipped with bilateral sensors worn throughout training measured overpressure in pounds per square inch (psi). On day 7, some participants experienced moderate blast exposure (peak pressure ≥5 psi). On day 10, 3 days post-exposure, blood was collected and compared to baseline with RNA-sequencing to establish gene expression changes. Based on dysregulation data from RNA-sequencing, followed by top gene networks identified with Ingenuity Pathway Analysis, a subset of genes was validated (NanoString). Five gene networks were dysregulated; specifically, two highly significant networks: (
1
) Cell Death and Survival (score: 42), including 70 genes, with 50 downregulated and (
2
) Cell Structure, Function, and Metabolism (score: 41), including 69 genes, with 41 downregulated. Genes related to ubiquitination, including neuronal development and repair: UPF1, RNA Helicase and ATPase (
UPF1
) was upregulated while UPF3 Regulator of Nonsense Transcripts Homolog B (
UPF3B
) was downregulated. Genes related to inflammation were upregulated, including AKT serine/threonine kinase 1 (
AKT1
), a gene coordinating cellular recovery following TBIs. Moderate blast exposure induced significant gene expression changes including gene networks involved in (
1
) cell death and survival and (
2
) cellular development and function. The present findings may have implications for understanding blast exposure pathology and subsequent recovery efforts.
Details
- Title: Subtitle
- A Moderate Blast Exposure Results in Dysregulated Gene Network Activity Related to Cell Death, Survival, Structure, and Metabolism
- Creators
- Katie A. Edwards - , , , , , , , , , , , , , , , , , ,Vida Motamedi - Wake Forest UniversityNicole D. Osier - The University of Texas at AustinHyung-Suk Kim - , , , , , , , , , , , , , , , , , ,Sijung Yun - National Institute of Nursing ResearchYoung-Eun Cho - University of IowaChen Lai - National Institute of Nursing ResearchKristine C. Dell - Walter Reed Army Institute of ResearchWalter Carr - Walter Reed Army Institute of ResearchPeter Walker - Naval Medical Research CommandStephen Ahlers - Naval Medical Research CommandMatthew LoPresti - Walter Reed Army Institute of ResearchAngela Yarnell - Walter Reed Army Institute of ResearchAnna Tschiffley - Naval Medical Research CommandJessica M. Gill - National Institute of Nursing Research
- Resource Type
- Journal article
- Publication Details
- Frontiers in neurology, Vol.11, pp.91-91
- DOI
- 10.3389/fneur.2020.00091
- PMID
- 32174881
- PMCID
- PMC7054450
- NLM abbreviation
- Front Neurol
- ISSN
- 1664-2295
- eISSN
- 1664-2295
- Publisher
- Frontiers Media S.A
- Grant note
- National Institute of Nursing Research NMRC#2011.0002; WRAIR#1796 / Medical Research and Materiel Command
- Language
- English
- Date published
- 02/26/2020
- Academic Unit
- Nursing; Fraternal Order of Eagles Diabetes Research Center
- Record Identifier
- 9984370633302771
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