A Multiwell Disc Appliance Used to Deliver Quantifiable Accelerations and Shear Stresses at Sonic Frequencies

Sarah Klemuk; Sarah Vigmostad; Kalyan Endapally; Andrew Wagner; Ingo Titze

doi:10.3390/pr2010071

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A Multiwell Disc Appliance Used to Deliver Quantifiable Accelerations and Shear Stresses at Sonic Frequencies

Journal article

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A Multiwell Disc Appliance Used to Deliver Quantifiable Accelerations and Shear Stresses at Sonic Frequencies

Sarah Klemuk, Sarah Vigmostad, Kalyan Endapally, Andrew Wagner and Ingo Titze

Processes, Vol.2(1), pp.71-88

01/10/2014

DOI: 10.3390/pr2010071

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Abstract

To mimic in vivo vibration of vocal fold cells, we studied the controllability and range of frequency, acceleration, duration, and shear stress in a new bioreactor attachment. The custom multiwell disc appliance fits into a commercially built rheometer, together termed a torsional rheometer bioreactor (TRB). Previous attachments to the TRB were capable of 50–100 Hz vibrations at relatively high strains but were limited to single-sample experiments. The TRB-multiwell disc system accommodates 20 samples in partially fluid-filled wells in an aseptic environment delivering three different acceleration conditions to different samples simultaneously. Frequency and amplitude used to calculate acceleration along with duration and shear stress were controllable and quantifiable using a combination of built-in rheometer sensors, manufacturer software, and smooth particle hydrodynamics (SPH) simulations. Computed shear stresses at the well bottom using SPH in two and three dimensions were verified with analytical approximations. Results demonstrate capabilities of the TRB-multiwell disc system that, when combined with computational modeling, provide quantifiable vibration parameters covering frequencies 0.01–250 Hz, accelerations of 0.02–300 m/s2, and shear stresses of 0.01–1.4 Pa. It is well-suited for studying cell function underlying vocal fold lamina propria homeostasis, inflammation, and wound healing under differential vibration conditions.

Details

Title: Subtitle: A Multiwell Disc Appliance Used to Deliver Quantifiable Accelerations and Shear Stresses at Sonic Frequencies
Creators: Sarah Klemuk
Sarah Vigmostad
Kalyan Endapally
Andrew Wagner
Ingo Titze
Resource Type: Journal article
Publication Details: Processes, Vol.2(1), pp.71-88
DOI: 10.3390/pr2010071
ISSN: 2227-9717
eISSN: 2227-9717
Language: English
Date published: 01/10/2014
Academic Unit: School of Music; Roy J. Carver Department of Biomedical Engineering; Communication Sciences and Disorders; Surgery; Mechanical Engineering
Record Identifier: 9984064578702771

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