Journal article
A Mutation Affecting Dihydropyridine-Sensitive Current Levels and Activation Kinetics in Drosophila Muscle and Mammalian Heart Calcium Channels
The Journal of neuroscience, Vol.18(7), pp.2335-2341
04/01/1998
DOI: 10.1523/JNEUROSCI.18-07-02335.1998
PMCID: PMC6793086
PMID: 9502794
Abstract
The
Dmca1D
gene encodes a
Drosophila
calcium channel α
1
subunit. We describe the first functional characterization of a mutation in this gene. This α
1
subunit mediates the dihydropyridine-sensitive calcium channel current in larval muscle but does not contribute to the amiloride-sensitive current in that tissue. A mutation, which changes a highly conserved Cys to Tyr in transmembrane domain IS1, identifies a residue important for channel function not only in
Drosophila
muscle but also in mammalian cardiac channels. In both cases, mutations in this Cys residue slow channel activation and reduce expressed currents. Amino acid substitutions at this Cys position in the cardiac α
1
subunit show that the size of the side chain, rather than its ability to form disulfide bonds, affects channel activation.
Details
- Title: Subtitle
- A Mutation Affecting Dihydropyridine-Sensitive Current Levels and Activation Kinetics in Drosophila Muscle and Mammalian Heart Calcium Channels
- Creators
- Dejian RenHongjian XuDaniel F EberlManinder ChopraLinda M Hall
- Resource Type
- Journal article
- Publication Details
- The Journal of neuroscience, Vol.18(7), pp.2335-2341
- Publisher
- Society for Neuroscience
- DOI
- 10.1523/JNEUROSCI.18-07-02335.1998
- PMID
- 9502794
- PMCID
- PMC6793086
- ISSN
- 0270-6474
- eISSN
- 1529-2401
- Language
- English
- Date published
- 04/01/1998
- Academic Unit
- Iowa Neuroscience Institute; Biology
- Record Identifier
- 9984070339402771
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