A Mutation in the FHA Domain of Coprinus cinereus Nbs1 Leads to Spo11-Independent Meiotic Recombination and Chromosome Segregation

K. Nicole Crown; Oleksandr P Savytskyy; Shehre-Banoo Malik; John Logsdon; R. Scott Williams; John A Tainer; Miriam E Zolan

doi:10.1534/g3.113.007906

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A Mutation in the FHA Domain of Coprinus cinereus Nbs1 Leads to Spo11-Independent Meiotic Recombination and Chromosome Segregation

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A Mutation in the FHA Domain of Coprinus cinereus Nbs1 Leads to Spo11-Independent Meiotic Recombination and Chromosome Segregation

K. Nicole Crown, Oleksandr P Savytskyy, Shehre-Banoo Malik, John Logsdon, R. Scott Williams, John A Tainer and Miriam E Zolan

G3 (Bethesda, Md.), Vol.3(11), pp.1927-1943

2013

DOI: 10.1534/g3.113.007906

PMCID: PMC3815056

PMID: 24062528

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Abstract

Nbs1, a core component of the Mre11-Rad50-Nbs1 complex, plays an essential role in the cellular response to DNA double-strand breaks (DSBs) and poorly understood roles in meiosis. We used the basidiomycete Coprinus cinereus to examine the meiotic roles of Nbs1. We identified the C. cinereus nbs1 gene and demonstrated that it corresponds to a complementation group previously known as rad3 . One allele, nbs1-2 , harbors a point mutation in the Nbs1 FHA domain and has a mild spore viability defect, increased frequency of meiosis I nondisjunction, and an altered crossover distribution. The nbs1-2 strain enters meiosis with increased levels of phosphorylated H2AX, which we hypothesize represent unrepaired DSBs formed during premeiotic replication. In nbs1-2 , there is no apparent induction of Spo11-dependent DSBs during prophase. We propose that replication-dependent DSBs, resulting from defective replication fork protection and processing by the Mre11-Rad50-Nbs1 complex, are competent to form meiotic crossovers in C. cinereus , and that these crossovers lead to high levels of faithful chromosome segregation. In addition, although crossover distribution is altered in nbs1-2 , the majority of crossovers were found in subtelomeric regions, as in wild-type. Therefore, the location of crossovers in C. cinereus is maintained when DSBs are induced via a Spo11-independent mechanism.

Coprinus cinereus

DNA replication

Investigations

meiosis

MRN complex

recombination

Details

Title: Subtitle: A Mutation in the FHA Domain of Coprinus cinereus Nbs1 Leads to Spo11-Independent Meiotic Recombination and Chromosome Segregation
Creators: K. Nicole Crown - Department of Biology, Indiana University, Bloomington, Indiana 47405
Oleksandr P Savytskyy - Department of Biology, Indiana University, Bloomington, Indiana 47405
Shehre-Banoo Malik - Department of Biology, University of Iowa, Iowa City, Iowa 52242
John Logsdon - Department of Biology, University of Iowa, Iowa City, Iowa 52242
R. Scott Williams - National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709
John A Tainer - The Scripps Research Institute, La Jolla, California 92037
Miriam E Zolan - Department of Biology, Indiana University, Bloomington, Indiana 47405
Resource Type: Journal article
Publication Details: G3 (Bethesda, Md.), Vol.3(11), pp.1927-1943
DOI: 10.1534/g3.113.007906
PMID: 24062528
PMCID: PMC3815056
NLM abbreviation: G3 (Bethesda)
ISSN: 2160-1836
eISSN: 2160-1836
Publisher: Genetics Society of America
Language: English
Date published: 2013
Academic Unit: Biology
Record Identifier: 9984217542902771

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