A New Targeted Transgenic Mouse Line for the Study of Protocadherin γC4

Cathy M McLeod; Camille M Hanes; Leah C Fuller; Samjhana Bhandari; Hannah G Lanthier; Robert W Burgess; Joshua A Weiner; Andrew M Garrett

doi:10.1002/dvg.70010

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A New Targeted Transgenic Mouse Line for the Study of Protocadherin γC4

Journal article

Peer reviewed

A New Targeted Transgenic Mouse Line for the Study of Protocadherin γC4

Cathy M McLeod, Camille M Hanes, Leah C Fuller, Samjhana Bhandari, Hannah G Lanthier, Robert W Burgess, Joshua A Weiner and Andrew M Garrett

Genesis (New York, N.Y. : 2000), Vol.63(1), e70010

02/2025

DOI: 10.1002/dvg.70010

PMCID: PMC11810047

PMID: 39923243

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https://pmc.ncbi.nlm.nih.gov/articles/PMC11810047/View

Open Access

Abstract

The γ-protocadherins (γ-Pcdhs) comprise 22 homophilic cell adhesion molecule isoforms, expressed from the Pcdhg gene cluster via promoter choice mechanisms that serve many crucial functions during neural development. Emerging evidence supports the hypothesis that distinct isoforms have unique functions. The γC4 isoform, which is expressed from the Pcdhgc4 promoter and includes its unique variable exon, is the sole γ-Pcdh isoform essential for the postnatal survival in mice. Here we describe a new mouse line (C4-GFP) in which Pcdhgc4 with a C-terminal GFP tag is expressed from the Rosa26 locus following excision of a lox-Stop-lox cassette by Cre recombinase. We report that restricted expression of this transgene in the nervous system using Nestin-Cre is sufficient to rescue the neonatal lethality of mice mutant for Pcdhgc4. This new line will be a vital tool for dissecting mechanisms underlying the functions of this essential cell adhesion molecule gene, mutations in which have been associated with neurodevelopmental disorders in humans.

Animals

Cadherin Related Proteins

Cadherins - genetics

Cadherins - metabolism

Green Fluorescent Proteins - genetics

Green Fluorescent Proteins - metabolism

Integrases - genetics

Integrases - metabolism

Mice

Mice, Transgenic

Promoter Regions, Genetic

Protein Isoforms - genetics

Protein Isoforms - metabolism

RNA, Untranslated

Details

Title: Subtitle: A New Targeted Transgenic Mouse Line for the Study of Protocadherin γC4
Creators: Cathy M McLeod - Wayne State University
Camille M Hanes - University of Iowa
Leah C Fuller - University of Iowa
Samjhana Bhandari - Wayne State University
Hannah G Lanthier - Wayne State University
Robert W Burgess - Jackson Laboratory
Joshua A Weiner - University of Iowa
Andrew M Garrett - Wayne State University
Resource Type: Journal article
Publication Details: Genesis (New York, N.Y. : 2000), Vol.63(1), e70010
DOI: 10.1002/dvg.70010
PMID: 39923243
PMCID: PMC11810047
NLM abbreviation: Genesis
ISSN: 1526-954X
eISSN: 1526-968X
Publisher: WILEY
Grant note: R01NS055272 / NINDS NIH HHS R01EY031690 / NEI NIH HHS R01 EY031690 / NEI NIH HHS Research to Prevent Blindness R01 NS055272 / NINDS NIH HHS P30EY004068 / NEI NIH HHS P30 EY004068 / NEI NIH HHS
Language: English
Date published: 02/2025
Academic Unit: Liberal Arts and Science Admin; Psychiatry; Iowa Neuroscience Institute; Biology
Record Identifier: 9984791078702771

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