Journal article
A Novel 2‐Hour Method for Rapid Preparation of Permanent Paraffin Sections When Treating Melanoma In Situ with Mohs Micrographic Surgery
Dermatologic Surgery, Vol.34(11), pp.1520-1526
11/2008
DOI: 10.1111/j.1524-4725.2008.34316.x
PMID: 18811716
Abstract
BACKGROUND Distinguishing sun‐induced melanocyte atypia from residual melanoma in situ (MIS) can be challenging, particularly when working with frozen sections. Immunostains such as melanoma‐associated antigen recognized by T cells (MART‐1) can assist, but paraffin sections provide an optimal means of analyzing melanocyte morphology. OBJECTIVE To verify the effectiveness of a 2‐hour paraffin processing technique that uses microwave technology in the preparation of MIS sections. METHODS Twelve MIS debulk specimens were divided into 4 pieces with each piece processed 1 of 4 ways: our 2‐hour paraffin technique with hematoxylin and eosin (H&E), conventional 24‐hour paraffin processing with H&E, frozen sections with H&E, and frozen sections with MART‐1 immunostaining. A Mohs surgeon and a dermatopathologist compared all specimens in a blinded fashion using a 3‐point ranking scale to assess ease of visualizing normal melanocytes, ease of visualizing abnormal melanocytes, and overall ability to adequately visualize epidermal and dermal structures. RESULTS A nonparametric signed rank test indicated no significant differences between our microwave technique and conventional paraffin processing in all 3 criteria (p=.29, .63, .75, respectively). Our microwave technique was significantly better than frozen H&E sections for all 3 criteria (p=.046, .004, .005, respectively). CONCLUSION This rapid microwave tissue processing technique is comparable with conventional paraffin section processing.
Details
- Title: Subtitle
- A Novel 2‐Hour Method for Rapid Preparation of Permanent Paraffin Sections When Treating Melanoma In Situ with Mohs Micrographic Surgery
- Creators
- Raj MallipeddiJeff StarkXian‐Jin XieMark MatthewsR Stan Taylor
- Resource Type
- Journal article
- Publication Details
- Dermatologic Surgery, Vol.34(11), pp.1520-1526
- Publisher
- Blackwell Publishing Inc; Malden, USA
- DOI
- 10.1111/j.1524-4725.2008.34316.x
- PMID
- 18811716
- ISSN
- 1076-0512
- eISSN
- 1524-4725
- Number of pages
- 7
- Language
- English
- Date published
- 11/2008
- Academic Unit
- Preventive and Community Dentistry; Biostatistics; Dental Research
- Record Identifier
- 9983917667702771
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