A Novel Bioreactor for Reconstitution of the Epithelium and Submucosal Glands in Decellularized Ferret Tracheas

Albert C Pai; Thomas J Lynch; Bethany A Ahlers; Vitaly Ievlev; John F Engelhardt; Kalpaj R Parekh

doi:10.3390/cells11061027

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A Novel Bioreactor for Reconstitution of the Epithelium and Submucosal Glands in Decellularized Ferret Tracheas

Journal article

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Peer reviewed

A Novel Bioreactor for Reconstitution of the Epithelium and Submucosal Glands in Decellularized Ferret Tracheas

Albert C Pai, Thomas J Lynch, Bethany A Ahlers, Vitaly Ievlev, John F Engelhardt and Kalpaj R Parekh

Cells (Basel, Switzerland), Vol.11(6), p.1027

03/18/2022

DOI: 10.3390/cells11061027

PMCID: PMC8947657

PMID: 35326478

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Abstract

Tracheal grafts introduce the possibility to treat airway pathologies that require resection. While there has been success with engraftment of the surface airway epithelium (SAE) onto decellularized tracheas, there has been minimal advancement in regenerating the submucosal glands (SMGs). We designed a cost-effective open-system perfusion bioreactor to investigate the engraftment potential of ferret SAEs and murine myoepithelial cells (MECs) on a partly decellularized ferret trachea with the goal of creating a fully functional tracheal replacement. An air-liquid interface was also arranged by perfusing humidified air through the lumen of a recellularized conduit to induce differentiation. Our versatile bioreactor design was shown to support the successful partial decellularization and recellularization of ferret tracheas. The decellularized grafts maintained biomechanical integrity and chondrocyte viability, consistent with other publications. The scaffolds supported SAE basal cell engraftment, and early differentiation was observed once an air-liquid interface had been established. Lastly, MEC engraftment was sustained, with evidence of diffuse SMG reconstitution. This model will help shed light on SMG regeneration and basal cell differentiation in vitro for the development of fully functional tracheal grafts before transplantation.

Animals

Bioreactors

Epithelial Cells

Epithelium

Ferrets

Mice

Trachea - surgery

Details

Title: Subtitle: A Novel Bioreactor for Reconstitution of the Epithelium and Submucosal Glands in Decellularized Ferret Tracheas
Creators: Albert C Pai - University of Iowa
Thomas J Lynch - University of Iowa
Bethany A Ahlers - University of Iowa
Vitaly Ievlev - University of Iowa
John F Engelhardt - University of Iowa
Kalpaj R Parekh - University of Iowa
Resource Type: Journal article
Publication Details: Cells (Basel, Switzerland), Vol.11(6), p.1027
DOI: 10.3390/cells11061027
PMID: 35326478
PMCID: PMC8947657
NLM abbreviation: Cells
ISSN: 2073-4409
eISSN: 2073-4409
Grant note: K08 HL114725 / NHLBI NIH HHS K99 HL155843 / NHLBI NIH HHS R01 DK047967 / NIDDK NIH HHS P30 DK054759 / NIDDK NIH HHS T32 HL007638 / NHLBI NIH HHS R01 HL136370 / NHLBI NIH HHS
Language: English
Date published: 03/18/2022
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Anatomy and Cell Biology; Radiation Oncology; Cardiothoracic Surgery; Internal Medicine
Record Identifier: 9984284327102771

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