A Novel Compound, "FA-1" Isolated from Prunus mume, Protects Human Bronchial Epithelial Cells and Keratinocytes from Cigarette Smoke Extract-Induced Damage

Andrew J Jang; Ji-Hyeok Lee; Mari Yotsu-Yamashita; Joodong Park; Steve Kye; Raymond L Benza; Michael J Passineau; You-Jin Jeon; Toru Nyunoya

doi:10.1038/s41598-018-29701-2

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A Novel Compound, "FA-1" Isolated from Prunus mume, Protects Human Bronchial Epithelial Cells and Keratinocytes from Cigarette Smoke Extract-Induced Damage

Journal article

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Peer reviewed

A Novel Compound, "FA-1" Isolated from Prunus mume, Protects Human Bronchial Epithelial Cells and Keratinocytes from Cigarette Smoke Extract-Induced Damage

Andrew J Jang, Ji-Hyeok Lee, Mari Yotsu-Yamashita, Joodong Park, Steve Kye, Raymond L Benza, Michael J Passineau, You-Jin Jeon and Toru Nyunoya

Scientific reports, Vol.8(1), pp.11504-10

07/31/2018

DOI: 10.1038/s41598-018-29701-2

PMID: 30065307

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Abstract

Extract of the Japanese apricot (JAE) has biological properties as an antioxidant and anti-inflammatory agent. We hypothesized that JAE might exert therapeutic effects on cigarette smoke (CS)-induced DNA damage and cytotoxicity. In this study, we found that concentrated JAE protects against cigarette smoke extract (CSE)-induced cytotoxicity and DNA damage accompanied by increased levels of aldehyde dehydrogenase (ALDH)2, 3A1, and Werner's syndrome protein (WRN) in immortalized human bronchial epithelial cells (HBEC2) and normal human epidermal keratinocytes (NHEK). Using the centrifugal partition chromatography (CPC) method, we identified an undescribed compound, 5-hydroxymethyl-2-furaldehyde bis(5-formylfurfuryl) acetal (which we named FA-1), responsible for the protective effects against CSE. This chemical structure has not been reported from a natural source to date. Protective effects of isolated FA-1 against CSE were observed in both HBEC2 and NHEK cells. The studies described herein suggest that FA-1 isolated from JAE protects against CSE-induced DNA damage and apoptosis by augmenting multiple isozymes of ALDH and DNA repair and reducing oxidative stress.

Aldehyde Dehydrogenase

Antioxidants - metabolism

Apoptosis - drug effects

Bronchi - cytology

Bronchi - drug effects

Cell Line

Cell Survival - drug effects

Cigarette Smoking - adverse effects

DNA Damage - drug effects

Epithelial Cells - drug effects

Epithelial Cells - metabolism

Furaldehyde - analogs & derivatives

Humans

Inflammation - metabolism

Keratinocytes - drug effects

Keratinocytes - metabolism

Nicotiana - chemistry

Oxidative Stress - drug effects

Plant Extracts - pharmacology

Protective Agents - pharmacology

Prunus - metabolism

Pulmonary Disease, Chronic Obstructive - metabolism

Smoke - adverse effects

Details

Title: Subtitle: A Novel Compound, "FA-1" Isolated from Prunus mume, Protects Human Bronchial Epithelial Cells and Keratinocytes from Cigarette Smoke Extract-Induced Damage
Creators: Andrew J Jang - Allegheny Health Network
Ji-Hyeok Lee - Lee Gil Ya Cancer and Diabetes Institute, 7-45, Songdodong, Yeonsugu, Incheon, 406-840, Republic of Korea
Mari Yotsu-Yamashita - Tohoku University
Joodong Park - Swedish Institute
Steve Kye - Acerta Pharma BV (Netherlands)
Raymond L Benza - Allegheny Health Network
Michael J Passineau - Allegheny Health Network
You-Jin Jeon - Jeju National University
Toru Nyunoya - University of Pittsburgh
Resource Type: Journal article
Publication Details: Scientific reports, Vol.8(1), pp.11504-10
DOI: 10.1038/s41598-018-29701-2
PMID: 30065307
NLM abbreviation: Sci Rep
ISSN: 2045-2322
eISSN: 2045-2322
Grant note: I01 CX001048 / CSRD VA
Language: English
Date published: 07/31/2018
Academic Unit: Internal Medicine
Record Identifier: 9985214173202771

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