Journal article
A Novel Impaction Technique to Create Experimental Articular Fractures in Large Animal Joints
Osteoarthritis and cartilage, Vol.21(1), pp.200-208
01/2013
DOI: 10.1016/j.joca.2012.10.004
PMCID: PMC3538937
PMID: 23069855
Abstract
Objective: A novel impaction fracture insult technique, developed for modeling post-traumatic osteoarthritis in porcine hocks in vivo, was tested to determine the extent to which it could replicate the cell-level cartilage pathology in human clinical intra-articular fractures.
Design: Eight fresh porcine hocks (whole-joint specimens with fully viable chondrocytes) were subjected to fracture insult. From the fractured distal tibial surfaces, osteoarticular fragments were immediately sampled and cultured in vitro for 48 h. These samples were analyzed for the distribution and progression of chondrocyte death, using the Live/Dead assay. Five control joints, in which "fractures" were simulated by means of surgical osteotomy, were also similarly analyzed.
Results: In the impaction-fractured joints, chondrocyte death was concentrated in regions adjacent to fracture lines (near-fracture regions), as evidenced by fractional cell death significantly higher (P < 0.0001) than in central non-fracture (control) regions. Although nominally similar spatial distribution patterns were identified in the osteotomized joints, fractional cell death in the near-osteotomy regions was nine-fold lower (P < 0.0001) than in the near-fracture regions. Cell death in the near-fracture regions increased monotonically during 48 h after impaction, dominantly within 1 mm from the fracture lines.
Conclusion: The impaction-fractured joints exhibited chondrocyte death characteristics reasonably consistent with those in human intra-articular fractures, but were strikingly different from those in "fractures" simulated by surgical osteotomy. These observations support promise of this new impaction fracture technique as a mechanical insult modality to replicate the pathophysiology of human intra-articular fractures in large animal joints in vivo.
Details
- Title: Subtitle
- A Novel Impaction Technique to Create Experimental Articular Fractures in Large Animal Joints
- Creators
- Yuki Tochigi - Department of Orthopaedics and Rehabilitation, University of IowaPeng Zhang - Department of Orthopaedics and Rehabilitation, University of Iowa, and Department of Orthopaedics, Affiliated Hospital of Shan Dong University of Traditional Chinese MedicineM. James Rudert - Department of Orthopaedics and Rehabilitation, University of IowaThomas E Baer - Department of Orthopaedics and Rehabilitation, University of IowaJames A Martin - Department of Orthopaedics and Rehabilitation, University of IowaStephen L Hillis - Department of Biostatistics, University of Iowa, and Center for Research in the Implementation of Innovative Strategies in Practice (CRIISP), VA Iowa City Medical CenterThomas D Brown - Department of Orthopaedics and Rehabilitation, and Department of Biomedical Engineering, University of Iowa
- Resource Type
- Journal article
- Publication Details
- Osteoarthritis and cartilage, Vol.21(1), pp.200-208
- DOI
- 10.1016/j.joca.2012.10.004
- PMID
- 23069855
- PMCID
- PMC3538937
- ISSN
- 1063-4584
- eISSN
- 1522-9653
- Grant note
- P50 AR055533 || AR / National Institute of Arthritis and Musculoskeletal and Skin Diseases : NIAMS
- Language
- English
- Date published
- 01/2013
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Radiology; Pharmaceutical Sciences and Experimental Therapeutics; Biostatistics; Orthopedics and Rehabilitation
- Record Identifier
- 9984040007302771
Metrics
34 Record Views