A Novel Model of Severe Gallstone Pancreatitis: Murine Pancreatic Duct Ligation Results in Systemic Inflammation and Substantial Mortality

Isaac Samuel; Zuobiao Yuan; David K Meyerholz; Erik Twait; Deborah E Williard; Duraisamy Kempuraj

doi:10.1159/000320776

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A Novel Model of Severe Gallstone Pancreatitis: Murine Pancreatic Duct Ligation Results in Systemic Inflammation and Substantial Mortality

Journal article

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Peer reviewed

A Novel Model of Severe Gallstone Pancreatitis: Murine Pancreatic Duct Ligation Results in Systemic Inflammation and Substantial Mortality

Isaac Samuel, Zuobiao Yuan, David K Meyerholz, Erik Twait, Deborah E Williard and Duraisamy Kempuraj

Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.], Vol.10(5), pp.536-544

12/2010

DOI: 10.1159/000320776

PMCID: PMC2992635

PMID: 20975317

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Abstract

Background: Suitable experimental models of gallstone pancreatitis with systemic inflammation and mortality are limited. We developed a novel murine model of duct-ligation-induced acute pancreatitis associated with multiorgan dysfunction and severe mortality. Methods: Laparotomy was done on C57/BL6 mice followed by pancreatic duct (PD) ligation, bile duct (BD) ligation without PD ligation, or sham operation. Results: Only mice with PD ligation developed acute pancreatitis and had 100% mortality. Pulmonary compliance was significantly reduced after PD ligation but not BD ligation. Bronchoalveolar lavage fluid neutrophil count and interleukin-1 β concentration, and the plasma creatinine level, were significantly elevated with PD ligation but not BD ligation. Pancreatic nuclearfactor kB (p65) and activator protein 1 (c-Jun) were activated within 1 h of PD ligation. Conclusion: PD-ligation-induced acute pancreatitis in mice is associated with systemic inflammation, acute lung injury, multiorgan dysfunction and death. The development of this novel model is an exciting and notable advance in the field.

Nuclear factor kB

Acute lung injury

Acute pancreatitis

Systemic inflammatory response syndrome

Mouse

Cytokines

Acinar cell

Multiple-organ dysfunction syndrome

Details

Title: Subtitle: A Novel Model of Severe Gallstone Pancreatitis: Murine Pancreatic Duct Ligation Results in Systemic Inflammation and Substantial Mortality
Creators: Isaac Samuel - Surgical Services, Iowa City Veterans Affairs Medical Center, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA
Zuobiao Yuan - Surgical Services, Iowa City Veterans Affairs Medical Center, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA
David K Meyerholz - Department of Pathology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA
Erik Twait - Surgical Services, Iowa City Veterans Affairs Medical Center, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA
Deborah E Williard - Surgical Services, Iowa City Veterans Affairs Medical Center, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA
Duraisamy Kempuraj - Surgical Services, Iowa City Veterans Affairs Medical Center, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA
Resource Type: Journal article
Publication Details: Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.], Vol.10(5), pp.536-544
Publisher: Elsevier B.V
DOI: 10.1159/000320776
PMID: 20975317
PMCID: PMC2992635
ISSN: 1424-3903
eISSN: 1424-3911
Language: English
Date published: 12/2010
Academic Unit: Pathology; IIHR--Hydroscience and Engineering; Surgery
Record Identifier: 9984051892702771

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