Journal article
A Novel Proteomics Approach to Identify Serum and Urinary Biomarkers and Pathways that Associate with Lower Urinary Tract Symptoms in Men and Women: Pilot Results of the Symptoms of Lower Urinary Tract Dysfunction Research Network (LURN) Study
Urology (Ridgewood, N.J.), Vol.129, pp.35-42
07/2019
DOI: 10.1016/j.urology.2019.03.014
PMCID: PMC6592754
PMID: 30922973
Abstract
To assess the feasibility of a novel proteomics approach to identify biomarkers associated with lower urinary tract symptoms (LUTS) within serum and urine, because many clinical factors contribute to LUTS in men and women. These factors confound clinicians' abilities to reliably evaluate and treat LUTS. Previous studies identified candidate LUTS biomarkers, but none are clinically utilized.
Eighteen male and 18 female symptoms of lower urinary tract dysfunction research network (LURN) observational cohort study participants with LUTS (measured on the LUTS Tool questionnaire) were randomly selected. Twelve male and 12 female controls with minimal or no LUTS were recruited and matched for clinico-demographic characteristics. The SomaScan Assay (SomaLogic) was used to measure the abundance of 1305 proteins contained within urine and serum. Statistical analyses were performed to evaluate reproducibility of assays, compare protein abundances, and estimate effect size.
SomaScan assay results were more reproducible in serum than in urine. Within serum, there were many more differentially abundant proteins between cases and controls in males than in females. An enrichment/pathway analysis of the affected proteins in male and female subjects demonstrated that the enriched Gene Ontology processes were related to prostate morphogenesis in men and growth and inflammation in women.
The pilot study results support that the etiology and pathophysiologic mechanisms underlying LUTS may be sex-specific. While further studies involving larger numbers of subjects are warranted, our results support the feasibility of a novel proteomic approach to identify biomarkers for diagnostic classification of LUTS.
Details
- Title: Subtitle
- A Novel Proteomics Approach to Identify Serum and Urinary Biomarkers and Pathways that Associate with Lower Urinary Tract Symptoms in Men and Women: Pilot Results of the Symptoms of Lower Urinary Tract Dysfunction Research Network (LURN) Study
- Creators
- Brian T Helfand - NorthShore University Health System, Evanston IL. Electronic address: bhelfand@northshore.orgVictor P Andreev - Arbor Research Collaborative for Health, Ann Arbor MINazema Y Siddiqui - Urogynecology & Reconstructive Pelvic Surgery, Duke University, Durham NCGang Liu - Arbor Research Collaborative for Health, Ann Arbor MIBradley A Erickson - Department of Urology, University of Iowa, Iowa City IAMargaret E Helmuth - Arbor Research Collaborative for Health, Ann Arbor MISusan K Lutgendorf - Psychological and Brain Sciences, The University of Iowa, Iowa City IAH Henry Lai - Washington University School of Medicine, St. Louis, MOZiya Kirkali - National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda MDLURN Study Group
- Resource Type
- Journal article
- Publication Details
- Urology (Ridgewood, N.J.), Vol.129, pp.35-42
- DOI
- 10.1016/j.urology.2019.03.014
- PMID
- 30922973
- PMCID
- PMC6592754
- NLM abbreviation
- Urology
- ISSN
- 0090-4295
- eISSN
- 1527-9995
- Publisher
- United States
- Grant note
- U01 DK100017 / NIDDK NIH HHS U01 DK100011 / NIDDK NIH HHS U01 DK097779 / NIDDK NIH HHS UL1 TR001422 / NCATS NIH HHS K23 DK110417 / NIDDK NIH HHS U01 DK097780 / NIDDK NIH HHS U01 DK099879 / NIDDK NIH HHS U01 DK097772 / NIDDK NIH HHS U01 DK097776 / NIDDK NIH HHS U01 DK099932 / NIDDK NIH HHS P30 CA086862 / NCI NIH HHS
- Language
- English
- Date published
- 07/2019
- Academic Unit
- Psychological and Brain Sciences; Iowa Neuroscience Institute; Obstetrics and Gynecology; Urology
- Record Identifier
- 9984002473502771
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