A Novel Role for UNC119 as an Enhancer of Synaptic Transmission

Katherine E Fehlhaber; Anurima Majumder; Kimberly K Boyd; Khris G Griffis; Nikolai O Artemyev; Gordon L Fain; Alapakkam P Sampath

doi:10.3390/ijms24098106

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A Novel Role for UNC119 as an Enhancer of Synaptic Transmission

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Peer reviewed

A Novel Role for UNC119 as an Enhancer of Synaptic Transmission

Katherine E Fehlhaber, Anurima Majumder, Kimberly K Boyd, Khris G Griffis, Nikolai O Artemyev, Gordon L Fain and Alapakkam P Sampath

International journal of molecular sciences, Vol.24(9), 8106

04/30/2023

DOI: 10.3390/ijms24098106

PMCID: PMC10178850

PMID: 37175812

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Abstract

Mammalian UNC119 is a ciliary trafficking chaperone highly expressed in the inner segment of retinal photoreceptors. Previous research has shown that UNC119 can bind to transducin, the synaptic ribbon protein RIBEYE, and the calcium-binding protein CaBP4, suggesting that UNC119 may have a role in synaptic transmission. We made patch-clamp recordings from retinal slices in mice with the gene deleted and showed that removal of even one gene of has no effect on the rod outer segment photocurrent, but acted on bipolar cells much like background light: it depolarized membrane potential, decreased sensitivity, accelerated response decay, and decreased the Hill coefficient of the response-intensity relationship. Similar effects were seen on rod bipolar-cell current and voltage responses, and after exposure to bright light to translocate transducin into the rod inner segment. These findings indicate that deletion reduces the steady-state glutamate release rate at rod synapses, though no change in the voltage dependence of the synaptic Ca current was detected. We conclude that UNC119, either by itself or together with transducin, can facilitate the release of glutamate at rod synapses, probably by some interaction with RIBEYE or other synaptic proteins rather than by binding to CaBP4 or calcium channels.

Animals

Glutamates - metabolism

Mammals - metabolism

Mice

Retina - metabolism

Synapses - metabolism

Synaptic Transmission - physiology

Transducin - metabolism

Details

Title: Subtitle: A Novel Role for UNC119 as an Enhancer of Synaptic Transmission
Creators: Katherine E Fehlhaber - Doheny Eye Institute
Anurima Majumder - University of Iowa
Kimberly K Boyd - University of Iowa
Khris G Griffis - Doheny Eye Institute
Nikolai O Artemyev - University of Iowa
Gordon L Fain - Doheny Eye Institute
Alapakkam P Sampath - Doheny Eye Institute
Resource Type: Journal article
Publication Details: International journal of molecular sciences, Vol.24(9), 8106
DOI: 10.3390/ijms24098106
PMID: 37175812
PMCID: PMC10178850
NLM abbreviation: Int J Mol Sci
ISSN: 1422-0067
eISSN: 1422-0067
Grant note: R01 EY01844 / NEI NIH HHS R01 EY10843 / NEI NIH HHS R01 EY12682 / NEI NIH HHS R01 EY28033 / NEI NIH HHS P30 EY00311 / NEI NIH HHS R01 EY29817 / NEI NIH HHS
Language: English
Date published: 04/30/2023
Academic Unit: Molecular Physiology and Biophysics; Iowa Neuroscience Institute; Ophthalmology and Visual Sciences
Record Identifier: 9984410860302771

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