Journal article
A Phosphatase Activity of Sts-1 Contributes to the Suppression of TCR Signaling
Molecular cell, Vol.27(3), pp.486-497
08/03/2007
DOI: 10.1016/j.molcel.2007.06.015
PMCID: PMC2709417
PMID: 17679096
Abstract
Precise signaling by the T cell receptor (TCR) is crucial for a proper immune response. To ensure that T cells respond appropriately to antigenic stimuli, TCR signaling pathways are subject to multiple levels of regulation. Sts-1 negatively regulates signaling pathways downstream of the TCR by an unknown mechanism(s). Here, we demonstrate that Sts-1 is a phosphatase that can target the tyrosine kinase Zap-70 among other proteins. The X-ray structure of the Sts-1 C terminus reveals that it has homology to members of the phosphoglycerate mutase/acid phosphatase (PGM/AcP) family of enzymes, with residues known to be important for PGM/AcP catalytic activity conserved in nature and position in Sts-1. Point mutations that impair Sts-1 phosphatase activity in vitro also impair the ability of Sts-1 to regulate TCR signaling in T cells. These observations reveal a PGM/AcP-like enzyme activity involved in the control of antigen receptor signaling.
Details
- Title: Subtitle
- A Phosphatase Activity of Sts-1 Contributes to the Suppression of TCR Signaling
- Creators
- Anatoly Mikhailik - Department of Molecular Genetics and Microbiology, Room 130, Life Sciences Building, Stony Brook University, Stony Brook, NY 11794-5222, USABradley Ford - Department of Physiology and Biophysics, Basic Sciences Tower, Stony Brook University, Stony Brook, NY 11794-8661, USAJames Keller - Department of Molecular Genetics and Microbiology, Room 130, Life Sciences Building, Stony Brook University, Stony Brook, NY 11794-5222, USAYunting Chen - Department of Physiology and Biophysics, Basic Sciences Tower, Stony Brook University, Stony Brook, NY 11794-8661, USANicolas Nassar - Department of Physiology and Biophysics, Basic Sciences Tower, Stony Brook University, Stony Brook, NY 11794-8661, USANick Carpino - Department of Molecular Genetics and Microbiology, Room 130, Life Sciences Building, Stony Brook University, Stony Brook, NY 11794-5222, USA
- Resource Type
- Journal article
- Publication Details
- Molecular cell, Vol.27(3), pp.486-497
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.molcel.2007.06.015
- PMID
- 17679096
- PMCID
- PMC2709417
- ISSN
- 1097-2765
- eISSN
- 1097-4164
- Language
- English
- Date published
- 08/03/2007
- Academic Unit
- Pathology
- Record Identifier
- 9984047771602771
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