Journal article
A Pilot Study Evaluating Combinatorial and Simultaneous Delivery of Polyethylenimine-Plasmid DNA Complexes Encoding for VEGF and PDGF for Bone Regeneration in Calvarial Bone Defects
Current pharmaceutical biotechnology, Vol.16(7), pp.655-660
2015
DOI: 10.2174/138920101607150427112753
PMCID: PMC4834972
PMID: 25934975
Abstract
Gene therapy is a promising strategy to deliver growth factors of interest locally in a sustained fashion and has the potential to overcome barriers to using recombinant protein therapy such as sustainability and cost. Recent studies demonstrate the safety and efficacy of non-viral delivery of plasmid DNA (pDNA) encoding a single growth factor to enhance bone healing. This pilot study is aimed at testing a non-viral gene delivery system that can deliver two different plasmids encoding two different growth factors. Polyethylenimine (PEI), a cationic polymer, was utilized as a gene delivery vector and collagen scaffold was used as a carrier to deliver the PEI-pDNA complexes encoding platelet derived growth factor B (PDGF-B) and/or vascular endothelial growth factor (VEGF). Calvarial defects in rats were implanted with scaffolds containing PEI-pPDGF-B complexes, PEI-pVEGF complexes or containing both PEI-pPDGF-B and PEI-pVEGF complexes in a 1:1 ratio of plasmids. The results indicated that bone regeneration as measured using micro-CT and histological assessments was inferior in groups treated with PEI-(pPDGF-B + pVEGF) complexes, compared to defects treated with PEI-pPDGF-B complexes. This pilot study that explores the feasibility and efficacy of combinatorial non-viral gene delivery system for bone regeneration appears to provide a rationale for investigation of sequential delivery of growth factors at specific time points during the healing phases and this will be explored further in future studies.
Details
- Title: Subtitle
- A Pilot Study Evaluating Combinatorial and Simultaneous Delivery of Polyethylenimine-Plasmid DNA Complexes Encoding for VEGF and PDGF for Bone Regeneration in Calvarial Bone Defects
- Creators
- Sheetal D'Mello - Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy, University of Iowa, Iowa City, IA, 52242Satheesh Elangovan - Department of Periodontics, College of Dentistry, University of Iowa, Iowa City, IA, 52242Liu Hong - Department of Prosthodontics, University of Iowa College of Dentistry, Iowa City, IA, USARyan D Ross - Department of Anatomy and Cell Biology, Rush Medical College, Chicago, IL, USAD. Rick Sumner - Department of Anatomy and Cell Biology, Rush Medical College, Chicago, IL, USAAliasger K Salem - Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy, University of Iowa, Iowa City, IA, 52242
- Resource Type
- Journal article
- Publication Details
- Current pharmaceutical biotechnology, Vol.16(7), pp.655-660
- DOI
- 10.2174/138920101607150427112753
- PMID
- 25934975
- PMCID
- PMC4834972
- NLM abbreviation
- Curr Pharm Biotechnol
- ISSN
- 1389-2010
- eISSN
- 1873-4316
- Language
- English
- Date published
- 2015
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics; Prosthodontics; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering; Periodontics
- Record Identifier
- 9984066088802771
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