Journal article
A Plastic Embedding Technique for Analyzing Fluorescent Dextran-Amine Labelled Neuronal Profiles
Biotechnic & histochemistry, Vol.67(3), pp.153-157
1992
DOI: 10.3109/10520299209110026
PMID: 1377506
Abstract
A plastic embedding technique employing fluorescently labelled dextran-amines is described. After application of tracer to cut nerves and appropriate transport time, animals were fixed in paraformaldehyde. Subsequently their brains were dissected, heads and brains were dehydrated, embedded in methacrylate and sectioned serially on a rotary microtome. Plastic sections allow high resolution of single neuron profiles and complete serial reconstruction of un-distorted sections, including embryos with large amounts of yolk. In conjunction with whole mount analysis and double labelling, this technique can accurately reveal the spatial relationships of nerve components throughout development.
Details
- Title: Subtitle
- A Plastic Embedding Technique for Analyzing Fluorescent Dextran-Amine Labelled Neuronal Profiles
- Creators
- B Fritzsch - 1Neurobiology Unit, Scripps Institution of Oceanography and Department of Neurosciences, A-001, University of California, San Diego La Jolla, California, 92093R. G Northcutt - 1Neurobiology Unit, Scripps Institution of Oceanography and Department of Neurosciences, A-001, University of California, San Diego La Jolla, California, 92093
- Resource Type
- Journal article
- Publication Details
- Biotechnic & histochemistry, Vol.67(3), pp.153-157
- DOI
- 10.3109/10520299209110026
- PMID
- 1377506
- NLM abbreviation
- Biotech Histochem
- ISSN
- 1052-0295
- eISSN
- 1473-7760
- Publisher
- Informa UK Ltd
- Language
- English
- Date published
- 1992
- Academic Unit
- Iowa Neuroscience Institute; Biology; Craniofacial Anomalies Research Center
- Record Identifier
- 9984070306602771
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