Journal article
A Preclinical Model to Assess Intestinal Barrier Integrity Using Canine Enteroids and Colonoids
Biology (Basel, Switzerland), Vol.14(3), 270
03/06/2025
DOI: 10.3390/biology14030270
PMCID: PMC11939752
PMID: 40136526
Abstract
While two-dimensional (2D) cell cultures, such as Caco-2 and Madin–Darby canine kidney (MDCK) cells are widely used in a variety of biological models, these two-dimensional in vitro systems present inherent limitations in replicating the complexities of in vivo biology. Recent progress in three-dimensional organoid technology has the potential to address these limitations. In this study, the characteristics of conventional 2D cell culture systems were compared to those of canine intestinal organoids (enteroids, ENT, and colonoids, COL). Light microscopy and transmission electron microscopy were employed to evaluate the microanatomy of ENT, COL, Caco-2, and MDCK cell monolayers, while transepithelial electrical resistance (TEER) values were measured to assess monolayer integrity. The TEER values of canine ENT monolayers more closely approximated reported TEER values for human small intestines compared to Caco-2 and MDCK monolayers. Additionally, canine ENT demonstrated greater monolayer stability than Caco-2 and MDCK cells. Notably, while all systems displayed desmosomes, canine ENT and COL exclusively produced mucus. These findings highlight the potential of the canine organoid system as a more biologically relevant model for in vitro studies, addressing the limitations of conventional 2D cell culture systems.
Details
- Title: Subtitle
- A Preclinical Model to Assess Intestinal Barrier Integrity Using Canine Enteroids and Colonoids
- Creators
- Megan P. Corbett - University of GeorgiaVojtech Gabriel - Iowa State UniversityVanessa Livania - Iowa State UniversityDavid Díaz-Regañón - Universidad Complutense de MadridAbigail RalstonChristopher Zdyrski - University of GeorgiaDongjie LiuSarah Minkler - Iowa State UniversityHannah Wickham - Iowa State UniversityAddison Lincoln - Iowa State UniversityKarel Paukner - Institute of Clinical and Experimental MedicineTodd Atherly - Iowa State UniversityMaria M. Merodio - Iowa State UniversityDipak Kumar Sahoo - Iowa State UniversityDavid K. Meyerholz - University of IowaKarin Allenspach - University of GeorgiaJonathan P. Mochel - University of Georgia
- Resource Type
- Journal article
- Publication Details
- Biology (Basel, Switzerland), Vol.14(3), 270
- DOI
- 10.3390/biology14030270
- PMID
- 40136526
- PMCID
- PMC11939752
- NLM abbreviation
- Biology (Basel)
- ISSN
- 2079-7737
- eISSN
- 2079-7737
- Publisher
- MDPI
- Grant note
- NSF SBIR Phase I: 1912948, FSU_0000022 NSF SBIR Phase I 18-550: Improving In Vitro Prediction of Oral Drug Permeability and Metabolism Using a Novel 3D Canine Organoid Model (NSF)
This research was funded by NSF SBIR Phase I 18-550: Improving In Vitro Prediction of Oral Drug Permeability and Metabolism Using a Novel 3D Canine Organoid Model (NSF: award number 1912948) and J.P.M. ISU Startup (FSU_0000022) funds.
- Language
- English
- Date published
- 03/06/2025
- Academic Unit
- Pathology
- Record Identifier
- 9984800203102771
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