Journal article
A ROS-dependent and Caspase-3-mediated apoptosis in sheep bronchial epithelial cells in response to Mycoplasma Ovipneumoniae infections
Veterinary immunology and immunopathology, Vol.187, pp.55-63
05/2017
DOI: 10.1016/j.vetimm.2017.04.004
PMID: 28494930
Abstract
Mycoplasma Ovipneumoniae (M. ovipneumoniae) is a primary etiological agent of enzootic pneumonia in sheep and goats. It can enter and colonize ovine respiratory epithelial cells to establish an infection, which leads a serious cell death of epithelial cells. However, the nature of the interaction between pathogen of M. ovipneumoniae and host cells in the cell injury is currently not well understood. In this study, we investigated the epithelial cell apoptosis caused by an infection of M. ovipneumoniae in sheep primary air-liquid interface (ALI) epithelial cultures. The results showed that M. ovipneumoniae could specifically bind to ciliated cells at early stage of infection. Flow cytometric analysis demonstrated that an infection of M. ovipneumoniae induced a time-dependent cell apoptotic cell death, accompanied with an increased production of extracellular nitric oxide (NO), intracellular reactive oxygen species (ROS) production and activation of caspase-3 signaling in sheep bronchial epithelial cells. The induced cell apoptosis was further confirmed by a transferase-mediated deoxyuridine triphosphate-biotin nick end-labeling (TUNEL) assay. Interestingly, the M. ovipneumoniae-induced apoptosis and activation of caspase-3 were correlated with the production of ROS but not NO. Mechanistically, M. ovipneumoniae-induced cell apoptosis was mediated by a mechanism by increasing the expression of phosphorylation of p38 and pro-apoptotic proteins, and activating caspase-3, caspase-8 and poly ADP-ribose polymerase (PARP) cleavage. These results suggest a ROS-dependent and caspase-3-mediated cell apoptosis in sheep bronchial epithelial cells in response to M. ovipneumoniae infections.
Details
- Title: Subtitle
- A ROS-dependent and Caspase-3-mediated apoptosis in sheep bronchial epithelial cells in response to Mycoplasma Ovipneumoniae infections
- Creators
- Di Xue - Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, ChinaYanan Li - Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, ChinaZhongjia Jiang - Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, ChinaGuangcun Deng - Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, ChinaMin Li - Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, ChinaXiaoming Liu - Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, ChinaYujiong Wang - Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, China
- Resource Type
- Journal article
- Publication Details
- Veterinary immunology and immunopathology, Vol.187, pp.55-63
- DOI
- 10.1016/j.vetimm.2017.04.004
- PMID
- 28494930
- NLM abbreviation
- Vet Immunol Immunopathol
- ISSN
- 0165-2427
- eISSN
- 1873-2534
- Publisher
- Elsevier B.V
- Grant note
- DOI: 10.13039/501100001809, name: National Natural Science Foundation of China, award: 31460660, 31572494
- Language
- English
- Date published
- 05/2017
- Academic Unit
- Anatomy and Cell Biology
- Record Identifier
- 9984288569602771
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