Journal article
A Rapid Way to Map Outheld-Wing Mutation in Drosophila melanogaster
Asian journal of animal and veterinary advances, Vol.6(3), pp.242-254
03/01/2011
DOI: 10.3923/ajava.2011.242.254
Abstract
Map-based positional cloning of Drosophila melanogaster genes is hampered by both the time-consuming, labor-intensive, error-prone nature of traditional methods for genetic mapping-SNP mapping is costly and P element mapping is low-resolution for limit number of P elements. Here we used two strategies to locate a spontaneous mutant screened in our lab. One is P element mapping to map the mutant in a 33 k-interval on the right arm of the second chromosome; another is combination of P element mapping and a Single-Nucleotide Polymorphism (SNP) mapping together to map the mutant in a very small interval, about 5 kb, on the right arm of the second chromosome efficiently. At last, we guessed that the mutant phenotype maybe resulted from the overexpression of twist gene. Comparing the two strategies, the method combining the P insertion mapping and SNP mapping could ward off their drawbacks. Therefore, the strategy of combination of the two methods provides a rapid protocol for mapping mutations.
Details
- Title: Subtitle
- A Rapid Way to Map Outheld-Wing Mutation in Drosophila melanogaster
- Creators
- Bo YuHuojun CaoChunjiang ZhaoChangxin WuXuemei Deng
- Resource Type
- Journal article
- Publication Details
- Asian journal of animal and veterinary advances, Vol.6(3), pp.242-254
- DOI
- 10.3923/ajava.2011.242.254
- ISSN
- 1683-9919
- eISSN
- 1996-3289
- Language
- English
- Date published
- 03/01/2011
- Academic Unit
- Anatomy and Cell Biology; Endodontics; Craniofacial Anomalies Research Center; Dental Research
- Record Identifier
- 9984066089002771
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